RecombinantRabMAb

Recombinant Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

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Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11934(B)] to PNK/PNKP
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-PNK/PNKP antibody [EPR11934(B)]
    See all PNK/PNKP primary antibodies

  • Description

    Rabbit monoclonal [EPR11934(B)] to PNK/PNKP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human PNK/PNKP aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96T60

  • Positive control

    • HeLa, Jurkat and PC3 cell lysates; Human testis lysate; Human kidney and pancreas tissues; PC3 cells.

  • General notes

     This product was previously labelled as PNK

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11934(B)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/250.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
Notes
ICC/IF
1/100 - 1/250.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.

    • Tissue specificity

      Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).

    • Involvement in disease

      Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.

    • Sequence similarities

      In the N-terminal section; belongs to the DNA 3' phosphatase family.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • 2''(3'')-polynucleotidase antibody
        • 2'(3')-polynucleotidase antibody
        • Bifunctional polynucleotide phosphatase/kinase antibody
        • DEM 1 antibody
        • DEM1 antibody
        • DNA 5' kinase/3' phosphatase antibody
        • DNA 5''-kinase/3''-phosphatase antibody
        • EIEE10 antibody
        • Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
        • MCSZ antibody
        • PNK 1 antibody
        • PNK1 antibody
        • Pnkp antibody
        • PNKP DNA kinase antibody
        • PNKP_HUMAN antibody
        • Polynucleotide 3'-phosphatase antibody
        • Polynucleotide 5' hydroxyl kinase antibody
        • Polynucleotide 5''-hydroxyl-kinase antibody
        • Polynucleotide kinase 3 prime phosphatase antibody
        • Polynucleotide kinase 3' phosphatase antibody
        • Polynucleotide Kinase antibody
        • Polynucleotide kinase-3''-phosphatase antibody
        see all

      Images

      • Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        All lanes : Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : PC3 cell lysate
        Lane 4 : Human testis lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 57 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        ab170954 showing +ve staining in Human testis tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        ab170954 showing +ve staining in Human normal pancreas tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        ab170954 showing +ve staining in Human thyroid gland carcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        Immunohistochemical analysis of paraffin-embedded Human prostate tissue, labeling PNK/PNKP using ab170954 at a 1/100 dilution.

        The use of an HRP/AP polymerized secondary antibody is recommended.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling PNK/PNKP using ab170954 at a 1/100 dilution.

        The use of an HRP/AP polymerized secondary antibody is recommended.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        Immunofluorescence analysis of PC3 cells, labeling PNK/PNKP using ab170954 at a 1/100 dilution.

      • Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

      References (1)

      Publishing research using ab170954? Please let us know so that we can cite the reference in this datasheet.

      ab170954 has been referenced in 1 publication.

      • Kalasova I  et al. Pathological mutations in PNKP trigger defects in DNA single-strand break repair but not DNA double-strand break repair. Nucleic Acids Res N/A:N/A (2020). PubMed: 32504494

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