For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    pnkpnkp-antibody-epr11934b-ab170954.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair DNA Damage Response Other
Share by email
RecombinantRabMAb

Recombinant Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
  • Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11934(B)] to PNK/PNKP
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human PNK/PNKP protein (ab131925)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-PNK/PNKP antibody [EPR11934(B)]
    See all PNK/PNKP primary antibodies
  • Description

    Rabbit monoclonal [EPR11934(B)] to PNK/PNKP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human PNK/PNKP aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96T60

  • Positive control

    • HeLa, Jurkat and PC3 cell lysates; Human testis lysate; Human kidney and pancreas tissues; PC3 cells.
  • General notes

     This product was previously labelled as PNK

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11934(B)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Other

Associated products

  • Alternative Versions

    • Anti-PNK/PNKP antibody [EPR11934(B)] - BSA and Azide free (ab249619)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • HeLa whole cell lysate (ab150035)
    • HeLa nuclear extract lysate (ab150036)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human PNK/PNKP protein (ab131925)
  • Related Products

    • Recombinant Human PNK/PNKP protein (ab131925)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/250.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
Notes
ICC/IF
1/100 - 1/250.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.
    • Tissue specificity

      Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
    • Involvement in disease

      Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.
    • Sequence similarities

      In the N-terminal section; belongs to the DNA 3' phosphatase family.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q96T60 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 11284 Human
      • Omim: 605610 Human
      • SwissProt: Q96T60 Human
      • Unigene: 78016 Human
      • Alternative names

        • 2''(3'')-polynucleotidase antibody
        • 2'(3')-polynucleotidase antibody
        • Bifunctional polynucleotide phosphatase/kinase antibody
        • DEM 1 antibody
        • DEM1 antibody
        • DNA 5' kinase/3' phosphatase antibody
        • DNA 5''-kinase/3''-phosphatase antibody
        • EIEE10 antibody
        • Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
        • MCSZ antibody
        • PNK 1 antibody
        • PNK1 antibody
        • Pnkp antibody
        • PNKP DNA kinase antibody
        • PNKP_HUMAN antibody
        • Polynucleotide 3'-phosphatase antibody
        • Polynucleotide 5' hydroxyl kinase antibody
        • Polynucleotide 5''-hydroxyl-kinase antibody
        • Polynucleotide kinase 3 prime phosphatase antibody
        • Polynucleotide kinase 3' phosphatase antibody
        • Polynucleotide Kinase antibody
        • Polynucleotide kinase-3''-phosphatase antibody
        see all

      Images

      • Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        All lanes : Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : PC3 cell lysate
        Lane 4 : Human testis lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 57 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        ab170954 showing +ve staining in Human testis tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        ab170954 showing +ve staining in Human normal pancreas tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        ab170954 showing +ve staining in Human thyroid gland carcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        Immunohistochemical analysis of paraffin-embedded Human prostate tissue, labeling PNK/PNKP using ab170954 at a 1/100 dilution.

        The use of an HRP/AP polymerized secondary antibody is recommended.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling PNK/PNKP using ab170954 at a 1/100 dilution.

        The use of an HRP/AP polymerized secondary antibody is recommended.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        Immunofluorescence analysis of PC3 cells, labeling PNK/PNKP using ab170954 at a 1/100 dilution.

      • Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

      Protocols

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab170954? Please let us know so that we can cite the reference in this datasheet.

      ab170954 has been referenced in 1 publication.

      • Kalasova I  et al. Pathological mutations in PNKP trigger defects in DNA single-strand break repair but not DNA double-strand break repair. Nucleic Acids Res N/A:N/A (2020). PubMed: 32504494

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab170954.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.