Recombinant
RabMAb

Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

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Overview

  • Product name
    Anti-PNK/PNKP antibody [EPR11934(B)]
    See all PNK/PNKP primary antibodies
  • Description
    Rabbit monoclonal [EPR11934(B)] to PNK/PNKP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human PNK/PNKP aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96T60

  • Positive control
    • HeLa, Jurkat and PC3 cell lysates; Human testis lysate; Human kidney and pancreas tissues; PC3 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11934(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function
      Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.
    • Tissue specificity
      Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
    • Involvement in disease
      Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.
    • Sequence similarities
      In the N-terminal section; belongs to the DNA 3' phosphatase family.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
      • Alternative names
        • 2''(3'')-polynucleotidase antibody
        • 2'(3')-polynucleotidase antibody
        • Bifunctional polynucleotide phosphatase/kinase antibody
        • DEM 1 antibody
        • DEM1 antibody
        • DNA 5' kinase/3' phosphatase antibody
        • DNA 5''-kinase/3''-phosphatase antibody
        • EIEE10 antibody
        • Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
        • MCSZ antibody
        • PNK 1 antibody
        • PNK1 antibody
        • Pnkp antibody
        • PNKP DNA kinase antibody
        • PNKP_HUMAN antibody
        • Polynucleotide 3'-phosphatase antibody
        • Polynucleotide 5' hydroxyl kinase antibody
        • Polynucleotide 5''-hydroxyl-kinase antibody
        • Polynucleotide kinase 3 prime phosphatase antibody
        • Polynucleotide kinase 3' phosphatase antibody
        • Polynucleotide Kinase antibody
        • Polynucleotide kinase-3''-phosphatase antibody
        see all

      Images

      • Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        All lanes : Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : PC3 cell lysate
        Lane 4 : Human testis lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 57 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        ab170954 showing +ve staining in Human testis tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        ab170954 showing +ve staining in Human normal pancreas tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        ab170954 showing +ve staining in Human thyroid gland carcinoma tissue.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        Immunohistochemical analysis of paraffin-embedded Human prostate tissue, labeling PNK/PNKP using ab170954 at a 1/100 dilution.

        The use of an HRP/AP polymerized secondary antibody is recommended.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling PNK/PNKP using ab170954 at a 1/100 dilution.

        The use of an HRP/AP polymerized secondary antibody is recommended.

      • Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        Immunofluorescence analysis of PC3 cells, labeling PNK/PNKP using ab170954 at a 1/100 dilution.

      References

      ab170954 has not yet been referenced specifically in any publications.

      Publishing research using ab170954? Please let us know so that we can cite the reference in this datasheet.

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