Recombinant
RabMAb

Recombinant Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

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Overview

  • Product name

    Anti-PNK/PNKP antibody [EPR11934(B)]
    See all PNK/PNKP primary antibodies

  • Description

    Rabbit monoclonal [EPR11934(B)] to PNK/PNKP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human PNK/PNKP aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96T60

  • Positive control

    • HeLa, Jurkat and PC3 cell lysates; Human testis lysate; Human kidney and pancreas tissues; PC3 cells.

  • General notes

     This product was previously labelled as PNK

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11934(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Function

      Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.

    • Tissue specificity

      Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).

    • Involvement in disease

      Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.

    • Sequence similarities

      In the N-terminal section; belongs to the DNA 3' phosphatase family.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • 2''(3'')-polynucleotidase antibody
        • 2'(3')-polynucleotidase antibody
        • Bifunctional polynucleotide phosphatase/kinase antibody
        • DEM 1 antibody
        • DEM1 antibody
        • DNA 5' kinase/3' phosphatase antibody
        • DNA 5''-kinase/3''-phosphatase antibody
        • EIEE10 antibody
        • Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
        • MCSZ antibody
        • PNK 1 antibody
        • PNK1 antibody
        • Pnkp antibody
        • PNKP DNA kinase antibody
        • PNKP_HUMAN antibody
        • Polynucleotide 3'-phosphatase antibody
        • Polynucleotide 5' hydroxyl kinase antibody
        • Polynucleotide 5''-hydroxyl-kinase antibody
        • Polynucleotide kinase 3 prime phosphatase antibody
        • Polynucleotide kinase 3' phosphatase antibody
        • Polynucleotide Kinase antibody
        • Polynucleotide kinase-3''-phosphatase antibody
        see all

      Images

      • Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Western blot - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        All lanes : Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : PC3 cell lysate
        Lane 4 : Human testis lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 57 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        ab170954 showing +ve staining in Human testis tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        ab170954 showing +ve staining in Human normal pancreas tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        ab170954 showing +ve staining in Human thyroid gland carcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        Immunohistochemical analysis of paraffin-embedded Human prostate tissue, labeling PNK/PNKP using ab170954 at a 1/100 dilution.

        The use of an HRP/AP polymerized secondary antibody is recommended.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling PNK/PNKP using ab170954 at a 1/100 dilution.

        The use of an HRP/AP polymerized secondary antibody is recommended.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)
        Immunocytochemistry/ Immunofluorescence - Anti-PNK/PNKP antibody [EPR11934(B)] (ab170954)

        Immunofluorescence analysis of PC3 cells, labeling PNK/PNKP using ab170954 at a 1/100 dilution.

      References

      Publishing research using ab170954? Please let us know so that we can cite the reference in this datasheet.

      ab170954 has not yet been referenced specifically in any publications.

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