Recombinant Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free (ab250354)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11935] to PNK/PNKP - BSA and Azide free
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PNK/PNKP antibody [EPR11935] - BSA and Azide free
See all PNK/PNKP primary antibodies -
Description
Rabbit monoclonal [EPR11935] to PNK/PNKP - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250354 is the carrier-free version of ab181107.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11935 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage. -
Tissue specificity
Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193). -
Involvement in disease
Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly. -
Sequence similarities
In the N-terminal section; belongs to the DNA 3' phosphatase family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 11284 Human
- Entrez Gene: 59047 Mouse
- Omim: 605610 Human
- SwissProt: Q96T60 Human
- SwissProt: Q9JLV6 Mouse
- Unigene: 78016 Human
- Unigene: 238254 Mouse
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Alternative names
- 2''(3'')-polynucleotidase antibody
- 2'(3')-polynucleotidase antibody
- Bifunctional polynucleotide phosphatase/kinase antibody
see all
Images
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All lanes : Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107) at 1/2000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Human testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?This data was developed using ab181107, the same antibody clone in a different buffer formulation.
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This data was developed using ab181107, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PNK/PNKP with ab181107 at 1/100 dilution. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab181107, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PNK/PNKP with ab181107 at 1/100 dilution. Counter stain Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?This data was developed using ab181107, the same antibody clone in a different buffer formulation.
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This data was developed using ab181107, the same antibody clone in a different buffer formulation.Western blot analysis of PNK/PNKP in immunoprecipitation pellets from HeLa cell lysate. ab181107 used at 1/20 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Right lane shows negative control.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250354 has not yet been referenced specifically in any publications.