Overview

  • Product name

    Anti-PNK/PNKP antibody [EPR11935] - C-terminal
    See all PNK/PNKP primary antibodies
  • Description

    Rabbit monoclonal [EPR11935] to PNK/PNKP - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    within Human PNK/PNKP aa 450 to the C-terminus. The exact sequence is proprietary.
    Database link: Q96T60

  • Positive control

    • HeLa, PC-3, Jurkat and Human testis lysate. Human breast and pancreas tissue. HeLa cells.
  • General notes

     

     

     This product was previously labelled as PNK

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11935
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
IP 1/20.

Target

  • Function

    Catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage.
  • Tissue specificity

    Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
  • Involvement in disease

    Defects in PNKP are the cause of microcephaly seizures and development delay (MCSZ) [MIM:613402]. It is characterized by microcephaly, infantile-onset seizures, severe intellectual disability and delayed motor milestones with absent speech or only achieving a few words. Most patients also have behavioral problems with hyperactivity. Microcephaly is progressive and without neuronal migration or structural abnormalities, consistent with primary microcephaly.
  • Sequence similarities

    In the N-terminal section; belongs to the DNA 3' phosphatase family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2''(3'')-polynucleotidase antibody
    • 2'(3')-polynucleotidase antibody
    • Bifunctional polynucleotide phosphatase/kinase antibody
    • DEM 1 antibody
    • DEM1 antibody
    • DNA 5' kinase/3' phosphatase antibody
    • DNA 5''-kinase/3''-phosphatase antibody
    • EIEE10 antibody
    • Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody
    • MCSZ antibody
    • PNK 1 antibody
    • PNK1 antibody
    • Pnkp antibody
    • PNKP DNA kinase antibody
    • PNKP_HUMAN antibody
    • Polynucleotide 3'-phosphatase antibody
    • Polynucleotide 5' hydroxyl kinase antibody
    • Polynucleotide 5''-hydroxyl-kinase antibody
    • Polynucleotide kinase 3 prime phosphatase antibody
    • Polynucleotide kinase 3' phosphatase antibody
    • Polynucleotide Kinase antibody
    • Polynucleotide kinase-3''-phosphatase antibody
    see all

Images

  • All lanes : Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107) at 1/2000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PNK/PNKP with ab181107 at 1/100 dilution. Counter stain Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PNK/PNKP with ab181107 at 1/100 dilution.  Counter stain Hematoxylin.

  • All lanes : Anti-PNK/PNKP antibody [EPR11935] - C-terminal (ab181107) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : PC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Western blot analysis of PNK/PNKP in immunoprecipitation pellets from HeLa cell lysate. ab181107 used at 1/20 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Right lane shows negative control.

References

ab181107 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions
Reduced Denaturing (4-12% Gradient Gel)
Loading amount
50 µg
Specification
U2OS cells
Blocking step
Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 15 2018

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (U2OS cells)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
U2OS cells

Abcam user community

Verified customer

Submitted Mar 15 2018

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