Overview

  • Product name

  • Description

    Rabbit polyclonal to PNMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 50 and 282 of Human PNMT (Uniprot ID P11086).

  • Positive control

    • Recombinant Human PNMT protein (ab89368) can be used as a positive control in WB. HepG2 cell lysates and U87 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab154282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded U87 xenograft labeling PNMT with ab154282 at 1/500.
  • Anti-PNMT antibody (ab154282) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 31 kDa

References

This product has been referenced in:

  • Albrecht H  et al. The Beta 2 Adrenergic Receptor Antagonist Timolol Improves Healing of Combined Burn and Radiation Wounds. Radiat Res 189:441-445 (2018). Read more (PubMed: 29373090) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154282.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up