Overview

  • Product name

  • Description

    Rabbit polyclonal to PNMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human PNMT aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab91000)

  • Positive control

    • This antibody gave a positive signal in Rat Adrenal gland Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab90862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).

Target

Images

  • Anti-PNMT antibody (ab90862) at 1 µg/ml + Adrenal gland (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 31 kDa
    Observed band size: 31 kDa


    Exposure time: 10 minutes

References

ab90862 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

I'm sorry but Kate is away today so I have issued your testing discount on her behalf.

DISCOUNT CODE: xxxxxxx

Expiration date: 2012-06-17

I am very pleased to hear you would like to accept our offer and test ab69579 in IHC with mouse samples. This code will give you 1 freeprimaryantibodybefore the expiration date. To redeem this offer, please submit an Abreview forIHC/mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site:

https://www.abcam.com/Abreviews

As this antibody has not previously been used for mouse or IHC I would suggest performing a western blot initially with your mouse samples as this would give an indication of if the antibody is able to recognise the mouse protein. As the immunogen used shares 94% homology with the mouse PNMT protein we would predict that is would but performing a western blot would help confirm it.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative.

If you place an order for this antibody before 4 pm today it should reach you by tomorrow.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here:

www.abcam.com/collaborationdiscount

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Answer

Thank you very much for your interest in PNMT antibodies ab69579 and ab90862.

As discussed on the telephone, to our knowledge these antibodies have not been tested in IHC-Fr or IHC-P. All tested and guaranteed applications are listed on the individual datasheets.

Therefore, I can offer a discount off a future purchase if you buy one of these antibodies now, test it in IHC-Fr or IHC-Pand submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and testone of these antibodies in IHC-P and IHC-Fr. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

2. Purchase the antibodyeither by phone, fax, or online (www.abcam.com).

3. Test it in IHC-P and IHC-Fr.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibody turns out to be unsuitable for IHC-P or IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Please let me know if you have any questions about this offer and I would be happy to help you further.

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