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  1. Link

    pnmt-antibody-epr75472-ab167427.pdf

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Neuroscience Neurotransmitter Biogenic Amines Noradrenaline
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RecombinantRabMAb

Recombinant Anti-PNMT antibody [EPR7547(2)] (ab167427)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (4)

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Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
  • Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)
  • Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
  • Anti-PNMT antibody [EPR7547(2)] (ab167427)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7547(2)] to PNMT
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Protein
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Recombinant Human PNMT protein (ab89368)

View more associated products

Overview

  • Product name

    Anti-PNMT antibody [EPR7547(2)]
    See all PNMT primary antibodies
  • Description

    Rabbit monoclonal [EPR7547(2)] to PNMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant Human PNMT protein (ab89368), K562 cell lysate and human adrenal gland and mouse kidney tissue lysates. ICC/IF: K562 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7547(2)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Adrenaline
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Catecholamines
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis

Associated products

  • Alternative Versions

    • Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free (ab249438)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)
    • Recombinant Human PNMT protein (ab89368)
  • Recombinant Protein

    • Recombinant Human PNMT protein (ab89368)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab167427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
ICC/IF
1/50 - 1/100.
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.

Target

  • Function

    Converts noradrenaline to adrenaline.
  • Pathway

    Catecholamine biosynthesis; (R)-adrenaline biosynthesis; (R)-adrenaline from (R)-noradrenaline: step 1/1.
  • Sequence similarities

    Belongs to the NNMT/PNMT/TEMT family.
  • Target information above from: UniProt accession P11086 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5409 Human
    • Entrez Gene: 18948 Mouse
    • Omim: 171190 Human
    • SwissProt: P11086 Human
    • SwissProt: P40935 Mouse
    • Unigene: 1892 Human
    • Unigene: 57030 Mouse
    • Alternative names

      • Noradrenaline N-methyltransferase antibody
      • PENT antibody
      • Phenylethanolamine N-methyltransferase antibody
      • PNMT antibody
      • PNMT_HUMAN antibody
      • PNMTase antibody
      see all

    Images

    • Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      All lanes : Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution

      Lane 1 : K562 lysate
      Lane 2 : Human adrenal gland lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 31 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Immunofluorescence analysis of K562 cells, labeling PNMT using ab167427 at a 1/50 dilution.
    • Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution + Mouse kidney tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 31 kDa
      Observed band size: 31 kDa


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Anti-PNMT antibody [EPR7547(2)] (ab167427)

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab167427? Please let us know so that we can cite the reference in this datasheet.

    ab167427 has been referenced in 4 publications.

    • Abid K  et al. The noradrenergic profile of plasma metanephrine in neuroblastoma patients is reproduced in xenograft mice models and arise from PNMT downregulation. Oncotarget 12:49-60 (2021). PubMed: 33456713
    • Scott AL  et al. Regulation of catecholamine release from the adrenal medulla is altered in deer mice (Peromyscus maniculatus) native to high altitudes. Am J Physiol Regul Integr Comp Physiol 317:R407-R417 (2019). PubMed: 31242021
    • Ni H  et al. Three-dimensional image reconstruction of distribution of Pnmt+cell-derived cells in murine heart. Sci Data 4:170134 (2017). IHC-Fr ; Mouse . PubMed: 28949324
    • Mahata SK  et al. Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells. J Endocrinol 230:309-23 (2016). PubMed: 27402067

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry free floating abreview for Anti-PNMT antibody [EPR7547(2)]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Adrenal gland)
    Specification
    Adrenal gland
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Andrew Jeffries

    Verified customer

    Submitted Apr 15 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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