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RecombinantRabMAb

Recombinant Anti-PNMT antibody [EPR7547(2)] (ab167427)

  • Datasheet
  • SDS
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Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
  • Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)
  • Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
  • Anti-PNMT antibody [EPR7547(2)] (ab167427)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7547(2)] to PNMT
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant Human PNMT protein (ab89368)

View more associated products

Overview

  • Product name

    Anti-PNMT antibody [EPR7547(2)]
    See all PNMT primary antibodies
  • Description

    Rabbit monoclonal [EPR7547(2)] to PNMT
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide, corresponding to residues in Human PNMT (Uniprot: P11086).

  • Positive control

    • WB: Recombinant Human PNMT protein (ab89368), K562 cell lysate and human adrenal gland and mouse kidney tissue lysates. ICC/IF: K562 cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7547(2)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Noradrenaline
    • Neuroscience
    • Neurotransmitter
    • Biogenic Amines
    • Adrenaline
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Catecholamines
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Hormone biosynthesis
    • Metabolism
    • Pathways and Processes
    • Endocrine metabolism
    • Hormone biosynthesis

Associated products

  • Alternative Versions

    • Anti-PNMT antibody [EPR7547(2)] - BSA and Azide free (ab249438)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)
    • Recombinant Human PNMT protein (ab89368)
  • Recombinant Protein

    • Recombinant Human PNMT protein (ab89368)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab167427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Mouse
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
ICC/IF
1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Converts noradrenaline to adrenaline.
    • Pathway

      Catecholamine biosynthesis; (R)-adrenaline biosynthesis; (R)-adrenaline from (R)-noradrenaline: step 1/1.
    • Sequence similarities

      Belongs to the NNMT/PNMT/TEMT family.
    • Target information above from: UniProt accession P11086 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5409 Human
      • Entrez Gene: 18948 Mouse
      • Omim: 171190 Human
      • SwissProt: P11086 Human
      • SwissProt: P40935 Mouse
      • Unigene: 1892 Human
      • Unigene: 57030 Mouse
      • Alternative names

        • Noradrenaline N-methyltransferase antibody
        • PENT antibody
        • Phenylethanolamine N-methyltransferase antibody
        • PNMT antibody
        • PNMT_HUMAN antibody
        • PNMTase antibody
        see all

      Images

      • Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
        Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
        All lanes : Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution

        Lane 1 : K562 lysate
        Lane 2 : Human adrenal gland lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 31 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)
        Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)
        Immunofluorescence analysis of K562 cells, labeling PNMT using ab167427 at a 1/50 dilution.
      • Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
        Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
        Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution + Mouse kidney tissue lysate at 20 µg

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 31 kDa
        Observed band size: 31 kDa


        Exposure time: 3 minutes


        Blocking and dilution buffer: 5% NFDM/TBST.

      • Anti-PNMT antibody [EPR7547(2)] (ab167427)
        Anti-PNMT antibody [EPR7547(2)] (ab167427)

      Protocols

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab167427? Please let us know so that we can cite the reference in this datasheet.

      ab167427 has been referenced in 2 publications.

      • Ni H  et al. Three-dimensional image reconstruction of distribution of Pnmt+cell-derived cells in murine heart. Sci Data 4:170134 (2017). IHC-Fr ; Mouse . PubMed: 28949324
      • Mahata SK  et al. Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells. J Endocrinol 230:309-23 (2016). PubMed: 27402067

      Customer reviews and Q&As

      Show All Reviews Q&A
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      There are currently no Customer reviews or Questions for ab167427.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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