RecombinantRabMAb

Recombinant Anti-PNMT antibody [EPR7547(2)] (ab167427)

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Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
  • Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)
  • Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
  • Anti-PNMT antibody [EPR7547(2)] (ab167427)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7547(2)] to PNMT
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PNMT antibody [EPR7547(2)]
    See all PNMT primary antibodies

  • Description

    Rabbit monoclonal [EPR7547(2)] to PNMT

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide, corresponding to residues in Human PNMT (Uniprot: P11086).

  • Positive control

    • WB: Recombinant Human PNMT protein (ab89368), K562 cell lysate and human adrenal gland and mouse kidney tissue lysates. ICC/IF: K562 cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab167427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Mouse
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
ICC/IF
1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    Images

    • Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      All lanes : Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution

      Lane 1 : K562 lysate
      Lane 2 : Human adrenal gland lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 31 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Immunofluorescence analysis of K562 cells, labeling PNMT using ab167427 at a 1/50 dilution.
    • Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution + Mouse kidney tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 31 kDa
      Observed band size: 31 kDa


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Anti-PNMT antibody [EPR7547(2)] (ab167427)

    References (2)

    Publishing research using ab167427? Please let us know so that we can cite the reference in this datasheet.

    ab167427 has been referenced in 2 publications.

    • Ni H  et al. Three-dimensional image reconstruction of distribution of Pnmt+cell-derived cells in murine heart. Sci Data 4:170134 (2017). IHC-Fr ; Mouse . PubMed: 28949324
    • Mahata SK  et al. Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells. J Endocrinol 230:309-23 (2016). PubMed: 27402067

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