Recombinant
RabMAb

Recombinant Anti-PNMT antibody [EPR7547(2)] (ab167427)

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Overview

  • Product name

    Anti-PNMT antibody [EPR7547(2)]
    See all PNMT primary antibodies

  • Description

    Rabbit monoclonal [EPR7547(2)] to PNMT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide, corresponding to residues in Human PNMT (Uniprot: P11086).

  • Positive control

    • WB: Recombinant Human PNMT protein (ab89368), K562 cell lysate and human adrenal gland and mouse kidney tissue lysates. ICC/IF: K562 cells.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab167427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    Images

    • Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      All lanes : Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution

      Lane 1 : K562 lysate
      Lane 2 : Human adrenal gland lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 31 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Immunofluorescence analysis of K562 cells, labeling PNMT using ab167427 at a 1/50 dilution.
    • Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)
      Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution + Mouse kidney tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 31 kDa
      Observed band size: 31 kDa


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

    References

    This product has been referenced in:

    • Ni H  et al. Three-dimensional image reconstruction of distribution of Pnmt+cell-derived cells in murine heart. Sci Data 4:170134 (2017). IHC-Fr ; Mouse . Read more (PubMed: 28949324) »
    • Mahata SK  et al. Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells. J Endocrinol 230:309-23 (2016). Read more (PubMed: 27402067) »
    See all 2 Publications for this product

    Publishing research using ab167427? Please let us know so that we can cite the reference in this datasheet.

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