Recombinant Anti-PNMT antibody [EPR7547(2)] (ab167427)
![Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)](https://www.abcam.com/ps/products/167/ab167427/Images/ab167427-165913-anti-pnmt-antibody-epr75472-western-blot.jpg "Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7547(2)] to PNMT
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-PNMT antibody [EPR7547(2)]
See all PNMT primary antibodies -
Description
Rabbit monoclonal [EPR7547(2)] to PNMT -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Recombinant Human PNMT protein (ab89368), K562 cell lysate and human adrenal gland and mouse kidney tissue lysates. ICC/IF: K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7547(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
|
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| ICC/IF |
1/50 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa. |
|
ICC/IF
1/50 - 1/100. |
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.
Target
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Function
Converts noradrenaline to adrenaline. -
Pathway
Catecholamine biosynthesis; (R)-adrenaline biosynthesis; (R)-adrenaline from (R)-noradrenaline: step 1/1. -
Sequence similarities
Belongs to the NNMT/PNMT/TEMT family. - Information by UniProt
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Database links
- Entrez Gene: 5409 Human
- Entrez Gene: 18948 Mouse
- Omim: 171190 Human
- SwissProt: P11086 Human
- SwissProt: P40935 Mouse
- Unigene: 1892 Human
- Unigene: 57030 Mouse
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Alternative names
- Noradrenaline N-methyltransferase antibody
- PENT antibody
- Phenylethanolamine N-methyltransferase antibody
see all
Images
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Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)All lanes : Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution
Lane 1 : K562 lysate
Lane 2 : Human adrenal gland lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 31 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)](https://www.abcam.com/ps/products/167/ab167427/Images/ab167427-165912-anti-pnmt-antibody-epr75472-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PNMT antibody [EPR7547(2)] (ab167427)Immunofluorescence analysis of K562 cells, labeling PNMT using ab167427 at a 1/50 dilution. -
![Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)](https://www.abcam.com/ps/products/167/ab167427/Images/ab167427-296646-anti-pnmt-antibody-epr75472-western-blot.jpg)
Western blot - Anti-PNMT antibody [EPR7547(2)] (ab167427)Anti-PNMT antibody [EPR7547(2)] (ab167427) at 1/1000 dilution + Mouse kidney tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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![Anti-PNMT antibody [EPR7547(2)] (ab167427)](https://www.abcam.com/ps/products/167/ab167427/Images/ab167427-4-benefits-of-recombinant-antibodies.png)
Anti-PNMT antibody [EPR7547(2)] (ab167427)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab167427 has been referenced in 4 publications.
- Abid K et al. The noradrenergic profile of plasma metanephrine in neuroblastoma patients is reproduced in xenograft mice models and arise from PNMT downregulation. Oncotarget 12:49-60 (2021). PubMed: 33456713
- Scott AL et al. Regulation of catecholamine release from the adrenal medulla is altered in deer mice (Peromyscus maniculatus) native to high altitudes. Am J Physiol Regul Integr Comp Physiol 317:R407-R417 (2019). PubMed: 31242021
- Ni H et al. Three-dimensional image reconstruction of distribution of Pnmt+cell-derived cells in murine heart. Sci Data 4:170134 (2017). IHC-Fr ; Mouse . PubMed: 28949324
- Mahata SK et al. Effect of heart failure on catecholamine granule morphology and storage in chromaffin cells. J Endocrinol 230:309-23 (2016). PubMed: 27402067