Overview

  • Product name

  • Description

    Rabbit polyclonal to PNN/DRSP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human PNN/DRSP aa 400-500 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab89383)

  • Positive control

    • This antibody gave a positive signal in Human Lung and Placenta tissue lysates.
  • General notes

    Previously labelled as Pinin. 

Properties

Applications

Our Abpromise guarantee covers the use of ab70605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 81 kDa).

Target

  • Function

    Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.
  • Tissue specificity

    Expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. Expressed strongly in melanoma metastasis lesions and advanced primary tumors.
  • Sequence similarities

    Belongs to the pinin family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus speckle. Cell junction > desmosome. Cell-cell contact area, predominantly desmosome of intercellular adherens junction. Not a nucleocytoplasmic shuttling protein.
  • Information by UniProt
  • Database links

  • Alternative names

    • 140 kDa nuclear and cell adhesion-related phosphoprotein antibody
    • Desmosome-associated protein antibody
    • Domain-rich serine protein antibody
    • DRS antibody
    • DRS protein antibody
    • DRSP antibody
    • Melanoma metastasis clone A protein antibody
    • MEMA antibody
    • Nuclear protein SDK3 antibody
    • Pinin antibody
    • PININ_HUMAN antibody
    • PNN antibody
    • SR like protein antibody
    • SR-like protein antibody
    see all

Images

  • All lanes : Anti-PNN/DRSP antibody (ab70605) at 1 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 81 kDa
    Observed band size: 87 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    PNN / DRSP contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight (87 kDa) than predicted (81 kDa).

References

ab70605 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab70605.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up