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    pnpla8-antibody-ab223726.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Lipases
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Anti-PNPLA8 antibody (ab223726)

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Western blot - Anti-PNPLA8 antibody (ab223726)
  • Immunocytochemistry/ Immunofluorescence - Anti-PNPLA8 antibody (ab223726)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNPLA8 antibody (ab223726)

Key features and details

  • Rabbit polyclonal to PNPLA8
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-PNPLA8 antibody
    See all PNPLA8 primary antibodies
  • Description

    Rabbit polyclonal to PNPLA8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human PNPLA8 aa 141-228.
    Sequence:

    DSGWLKQKNIKQAIKSLKKYSDKSAEKSPFPEEKSHIIDKEEDIGKRSLF HYTSSITTKFGDSFYFLSNHINSYFKRKEKMSQQKENE


    Database link: Q9NP80
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: U-2197 cell lysate. ICC/IF: U-2 OS cells. HC-P: Human duodenum tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Lipases
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Lipid Phosphatases
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab223726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 88 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function

    Calcium-independent phospholipase A2, which catalyzes the hydrolysis of the sn-2 position of glycerophospholipids, PtdSer and to a lower extent PtdCho. Cleaves membrane phospholipids.
  • Tissue specificity

    Expressed in parenchymal tissues including heart, skeletal muscle, placenta, brain, liver and pancreas. Also expressed in bronchial epithelial cells and kidney. Highest expression is observed in skeletal muscle and heart.
  • Sequence similarities

    Contains 1 patatin domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasm > perinuclear region.
  • Target information above from: UniProt accession Q9NP80 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 50640 Human
    • Omim: 612123 Human
    • SwissProt: Q9NP80 Human
    • Unigene: 617340 Human
    • Unigene: 732217 Human
    • Alternative names

      • Calcium-independent phospholipase A2-gamma antibody
      • Intracellular membrane associated calcium independent phospholipase A2 gamma antibody
      • Intracellular membrane-associated calcium-independent phospholipase A2 gamma antibody
      • IPLA2 2 antibody
      • iPLA2 gamma antibody
      • IPLA2(GAMMA) antibody
      • iPLA2-2 antibody
      • iPLA2-gamma antibody
      • IPLA22 antibody
      • IPLA2G antibody
      • Patatin like phospholipase domain containing protein 8 antibody
      • Patatin-like phospholipase domain-containing protein 8 antibody
      • PLPL8_HUMAN antibody
      • PNPLA-gamma antibody
      • PNPLA8 antibody
      see all

    Images

    • Western blot - Anti-PNPLA8 antibody (ab223726)
      Western blot - Anti-PNPLA8 antibody (ab223726)
      Anti-PNPLA8 antibody (ab223726) at 1/100 dilution + U-2197 cell lysate

      Predicted band size: 88 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PNPLA8 antibody (ab223726)
      Immunocytochemistry/ Immunofluorescence - Anti-PNPLA8 antibody (ab223726)

      PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PNPLA8 (green) using ab223726 (4 µg/ml) in ICC/IF.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNPLA8 antibody (ab223726)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNPLA8 antibody (ab223726)

      Paraffin embedded human duodenum tissue stained for PNPLA8 with ab223726 (1/50 dilution) in immunohistochemical analysis.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab223726? Please let us know so that we can cite the reference in this datasheet.

    ab223726 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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