Product nameAnti-PNPT1 antibody
See all PNPT1 primary antibodies
DescriptionRabbit polyclonal to PNPT1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Ferret, Macaque monkey, Rhesus monkey, Common marmoset
- 293T, HeLa and Jurkat whole cell lysate (ab7899).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 86 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevancePNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation. It contains one KH domain and one S1 motif domain.
- 3' 5' RNA exonuclease antibody
- COXPD13 antibody
- DFNB70 antibody
All lanes : Anti-PNPT1 antibody (ab157109) at 0.4 µg/ml
Lane 1 : 293T cell lysate at 50 µg
Lane 2 : 293T cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 3 minutes
Detection of PNPT1 by Western blot of immunoprecipitate. Immunoprecipitation of HeLa whole cell lysate at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1 : IP with an alternative Rabbit Anti-PNPT1 antibody.
Lane 2 : IP with ab157109.
Lane 3 : Control IgG.
The subsequent Western blot of immunoprecipitates was done using ab157109 at 1 µg/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-PNPT1 antibody (ab157109)
All lanes : 293T whole cell lysate
This product has been referenced in:
- Stefan-Lifshitz M et al. Epigenetic modulation of ß cells by interferon-a via PNPT1/mir-26a/TET2 triggers autoimmune diabetes. JCI Insight 4:N/A (2019). Read more (PubMed: 30721151) »
- Nejad C et al. miR-222 isoforms are differentially regulated by type-I interferon. RNA 24:332-341 (2018). Read more (PubMed: 29263133) »