Anti-PNPT1 antibody (ab157109)
Key features and details
- Rabbit polyclonal to PNPT1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PNPT1 antibody
See all PNPT1 primary antibodies -
Description
Rabbit polyclonal to PNPT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Ferret, Macaque monkey, Rhesus monkey, Common marmoset
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 700-750 of Human PNPT1 (NP_149100.2).
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Positive control
- 293T, HeLa and Jurkat whole cell lysate (ab7899).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab157109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 86 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Relevance
PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation. It contains one KH domain and one S1 motif domain. -
Cellular localization
Mitochondrion -
Database links
- Entrez Gene: 100393231 Common marmoset
- Entrez Gene: 481376 Dog
- Entrez Gene: 100052355 Horse
- Entrez Gene: 87178 Human
- Entrez Gene: 71701 Mouse
- Entrez Gene: 100642182 Pig
- Entrez Gene: 360992 Rat
- GenBank: NP_149100.2 Human
see all -
Alternative names
- 3' 5' RNA exonuclease antibody
- COXPD13 antibody
- DFNB70 antibody
see all
Images
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Western blot - Anti-PNPT1 antibody (ab157109)All lanes : Anti-PNPT1 antibody (ab157109) at 0.4 µg/ml
Lane 1 : 293T cell lysate at 50 µg
Lane 2 : 293T cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-PNPT1 antibody (ab157109)Detection of PNPT1 by Western blot of immunoprecipitate. Immunoprecipitation of HeLa whole cell lysate at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1 : IP with an alternative Rabbit Anti-PNPT1 antibody.
Lane 2 : IP with ab157109.
Lane 3 : Control IgG.
The subsequent Western blot of immunoprecipitates was done using ab157109 at 1 µg/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-PNPT1 antibody (ab157109)
All lanes : 293T whole cell lysate
Protocols
Datasheets and documents
References (3)
ab157109 has been referenced in 3 publications.
- Stefan-Lifshitz M et al. Epigenetic modulation of ß cells by interferon-a via PNPT1/mir-26a/TET2 triggers autoimmune diabetes. JCI Insight 4:N/A (2019). PubMed: 30721151
- Nejad C et al. miR-222 isoforms are differentially regulated by type-I interferon. RNA 24:332-341 (2018). PubMed: 29263133
- Li D et al. Long Noncoding RNA pancEts-1 Promotes Neuroblastoma Progression through hnRNPK-Mediated ß-Catenin Stabilization. Cancer Res 78:1169-1183 (2018). PubMed: 29311158
