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    pnpt1-antibody-ab157109.pdf

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Immunology Innate Immunity Cytokines Interferons
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Anti-PNPT1 antibody (ab157109)

  • Datasheet
Submit a review Submit a question References (3)

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Western blot - Anti-PNPT1 antibody (ab157109)
  • Immunoprecipitation - Anti-PNPT1 antibody (ab157109)

Key features and details

  • Rabbit polyclonal to PNPT1
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-PNPT1 antibody
    See all PNPT1 primary antibodies
  • Description

    Rabbit polyclonal to PNPT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Ferret, Macaque monkey, Rhesus monkey, Common marmoset
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 700-750 of Human PNPT1 (NP_149100.2).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293T, HeLa and Jurkat whole cell lysate (ab7899).
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human PNPT1 protein (ab202628)

Applications

Our Abpromise guarantee covers the use of ab157109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 86 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation. It contains one KH domain and one S1 motif domain.
  • Cellular localization

    Mitochondrion
  • Database links

    • Entrez Gene: 100393231 Common marmoset
    • Entrez Gene: 481376 Dog
    • Entrez Gene: 100052355 Horse
    • Entrez Gene: 87178 Human
    • Entrez Gene: 71701 Mouse
    • Entrez Gene: 100642182 Pig
    • Entrez Gene: 360992 Rat
    • GenBank: NP_149100.2 Human
    • Omim: 610316 Human
    • SwissProt: Q8TCS8 Human
    • SwissProt: Q8K1R3 Mouse
    see all
  • Alternative names

    • 3' 5' RNA exonuclease antibody
    • COXPD13 antibody
    • DFNB70 antibody
    • old-35 antibody
    • OLD35 antibody
    • PNPASE antibody
    • PNPase 1 antibody
    • Polynucleotide phosphorylase like protein antibody
    • Polyribonucleotide nucleotidyltransferase 1, mitochondrial antibody
    see all

Images

  • Western blot - Anti-PNPT1 antibody (ab157109)
    Western blot - Anti-PNPT1 antibody (ab157109)
    All lanes : Anti-PNPT1 antibody (ab157109) at 0.4 µg/ml

    Lane 1 : 293T cell lysate at 50 µg
    Lane 2 : 293T cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 50 µg
    Lane 4 : Jurkat cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 86 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-PNPT1 antibody (ab157109)
    Immunoprecipitation - Anti-PNPT1 antibody (ab157109)
    Detection of PNPT1 by Western blot of immunoprecipitate. Immunoprecipitation of HeLa whole cell lysate at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), using the following:
    Lane 1 : IP with an alternative Rabbit Anti-PNPT1 antibody.
    Lane 2 : IP with ab157109.
    Lane 3 : Control IgG.
    The subsequent Western blot of immunoprecipitates was done using ab157109 at 1 µg/ml.
    Detection: Chemiluminescence with exposure time of 30 seconds.

    All lanes : Anti-PNPT1 antibody (ab157109)

    All lanes : 293T whole cell lysate

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab157109? Please let us know so that we can cite the reference in this datasheet.

    ab157109 has been referenced in 3 publications.

    • Stefan-Lifshitz M  et al. Epigenetic modulation of ß cells by interferon-a via PNPT1/mir-26a/TET2 triggers autoimmune diabetes. JCI Insight 4:N/A (2019). PubMed: 30721151
    • Nejad C  et al. miR-222 isoforms are differentially regulated by type-I interferon. RNA 24:332-341 (2018). PubMed: 29263133
    • Li D  et al. Long Noncoding RNA pancEts-1 Promotes Neuroblastoma Progression through hnRNPK-Mediated ß-Catenin Stabilization. Cancer Res 78:1169-1183 (2018). PubMed: 29311158

    Customer reviews and Q&As

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