Anti-PNPT1 antibody (ab157109)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-PNPT1 antibody
See all PNPT1 primary antibodies -
Description
Rabbit polyclonal to PNPT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Ferret, Macaque monkey, Rhesus monkey, Common marmoset
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 700-750 of Human PNPT1 (NP_149100.2).
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Positive control
- 293T, HeLa and Jurkat whole cell lysate (ab7899).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab157109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 86 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Relevance
PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation. It contains one KH domain and one S1 motif domain. -
Cellular localization
Mitochondrion -
Database links
- Entrez Gene: 100393231 Common marmoset
- Entrez Gene: 481376 Dog
- Entrez Gene: 100052355 Horse
- Entrez Gene: 87178 Human
- Entrez Gene: 71701 Mouse
- Entrez Gene: 100642182 Pig
- Entrez Gene: 360992 Rat
- GenBank: NP_149100.2 Human
see all -
Alternative names
- 3' 5' RNA exonuclease antibody
- COXPD13 antibody
- DFNB70 antibody
see all
Images
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Western blot - Anti-PNPT1 antibody (ab157109)All lanes : Anti-PNPT1 antibody (ab157109) at 0.4 µg/ml
Lane 1 : 293T cell lysate at 50 µg
Lane 2 : 293T cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-PNPT1 antibody (ab157109)Detection of PNPT1 by Western blot of immunoprecipitate. Immunoprecipitation of HeLa whole cell lysate at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane), using the following:
Lane 1 : IP with an alternative Rabbit Anti-PNPT1 antibody.
Lane 2 : IP with ab157109.
Lane 3 : Control IgG.
The subsequent Western blot of immunoprecipitates was done using ab157109 at 1 µg/ml.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-PNPT1 antibody (ab157109)
All lanes : 293T whole cell lysate
Datasheets and documents
References
This product has been referenced in:
- Stefan-Lifshitz M et al. Epigenetic modulation of ß cells by interferon-a via PNPT1/mir-26a/TET2 triggers autoimmune diabetes. JCI Insight 4:N/A (2019). Read more (PubMed: 30721151) »
- Nejad C et al. miR-222 isoforms are differentially regulated by type-I interferon. RNA 24:332-341 (2018). Read more (PubMed: 29263133) »
