• Product name

  • Description

    Rabbit polyclonal to PNUTS
  • Host species

  • Specificity

    We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Other Immunogen Type corresponding to PNUTS aa 350-400.

  • Positive control

    • Nuclear extract from Hela cells
  • General notes

     This product was previously labelled as PPP1R10




Our Abpromise guarantee covers the use of ab70247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48, 115 kDa (predicted molecular weight: 99 kDa).
IP Use a concentration of 1 - 10 µg/ml.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function

    Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers.
  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Contains 1 C3H1-type zinc finger.
    Contains 1 TFIIS N-terminal domain.
  • Post-translational

    Phosphorylated on Ser-398 by PKA within the region necessary for interaction with PPP1CA.
  • Cellular localization

    Nucleus. Found in discrete nucleoplasmic bodies and within nucleoli. Associates with chromatin during interphase, excluded from condensed chromosomes during early mitosis and is reloaded onto chromosomes at the late telophase (By similarity).
  • Information by UniProt
  • Database links

  • Alternative names

    • CAT 53 antibody
    • CAT53 antibody
    • FB 19 antibody
    • FB19 antibody
    • FB19 protein antibody
    • HLA-C associated transcript 53 antibody
    • MHC class I region proline rich protein CAT53 antibody
    • MHC class I region proline-rich protein CAT53 antibody
    • p99 antibody
    • Phosphatase 1 nuclear targeting subunit antibody
    • Phosphatase nuclear targeting subunit antibody
    • PNUTS antibody
    • PP1 binding protein of 114 kDa antibody
    • PP1 nuclear targeting subunit antibody
    • PP1-binding protein of 114 kDa antibody
    • PP1R10 antibody
    • PP1RA_HUMAN antibody
    • ppp1r10 antibody
    • Protein FB19 antibody
    • Protein phosphatase 1 nuclear targeting subunit antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 10 antibody
    • Protein phosphatase 1 regulatory subunit 10 antibody
    • R111 antibody
    • Serine/threonine protein phosphatase 1 regulatory subunit 10 antibody
    • Serine/threonine-protein phosphatase 1 regulatory subunit 10 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PNUTS with ab70247 at 1/1000 (1µg/ml). Detection: DAB.

  • Lanes 1-2 : Anti-PNUTS antibody (ab70247) at 1 µg/ml
    Lanes 3-4 : Anti-PNUTS antibody (ab70247) at 0.2 µg/ml

    Lanes 1 & 3 : Nuclear extract from Hela cells at 15 µg
    Lanes 2 & 4 : Nuclear extract from Hela cells at 45 µg

    Predicted band size: 99 kDa
    Observed band size: 115 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.

  • Immunoprecipitation/ Western Blot of PNUTS Lane 1: Another antibody which recognizes a different epitope of PNUTS. Lane 2: Another antibody which recognizes a different epitope of PNUTS. Lane 3: ab70247 at 1µg/100µg nuclear extract. Lane 4: Another antibody which recognizes a different epitope of PNUTS. Detection: Chemiluminescence with exposure times of 3 seconds.


ab70247 has not yet been referenced specifically in any publications.

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