Key features and details
- Rabbit polyclonal to PNUTS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PNUTS antibody
See all PNUTS primary antibodies
DescriptionRabbit polyclonal to PNUTS
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish
Other Immunogen Type corresponding to PNUTS (C terminal). A region between residue 900 and the C-terminus (residue 940) of human PNUTS.
- Nuclear extract of Hela cells
This product was previously labelled as PPP1R10
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to PNUTS immobilized on solid support.
Our Abpromise guarantee covers the use of ab70248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 115 kDa (predicted molecular weight: 99 kDa).|
|IP||Use at 1-10 µg/mg of lysate.|
FunctionScaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers.
Tissue specificityExpressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesContains 1 C3H1-type zinc finger.
Contains 1 TFIIS N-terminal domain.
modificationsPhosphorylated on Ser-398 by PKA within the region necessary for interaction with PPP1CA.
Cellular localizationNucleus. Found in discrete nucleoplasmic bodies and within nucleoli. Associates with chromatin during interphase, excluded from condensed chromosomes during early mitosis and is reloaded onto chromosomes at the late telophase (By similarity).
- Information by UniProt
- CAT 53 antibody
- CAT53 antibody
- FB 19 antibody
Lanes 1-2 : Anti-PNUTS antibody (ab70248) at 1 µg/ml
Lanes 3-4 : Anti-PNUTS antibody (ab70248) at 0.2 µg/ml
Lanes 1 & 3 : Nuclear extracts from Hela cells at 15 µg
Lanes 2 & 4 : Nuclear extracts from Hela cells at 45 µg
Predicted band size: 99 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Immunoprecipitation/ Western Blot of PNUTS Lane 1: Another antibody which recognizes a different epitope of PNUTS. Lane 2: Another antibody which recognizes a different epitope of PNUTS. Lane 3: Another antibody which recognizes a different epitope of PNUTS. Lane 4: ab70248 at 1µg/100µg nuclear extract. Detection: Chemiluminescence with exposure times of 3 seconds.
ab70248 has not yet been referenced specifically in any publications.