Overview

  • Product name

  • Description

    Rabbit polyclonal to PNUTS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Medaka fish
  • Immunogen

    Other Immunogen Type corresponding to PNUTS (C terminal). A region between residue 900 and the C-terminus (residue 940) of human PNUTS.

  • Positive control

    • Nuclear extract of Hela cells
  • General notes

     This product was previously labelled as PPP1R10

     

Properties

Applications

Our Abpromise guarantee covers the use of ab70248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 115 kDa (predicted molecular weight: 99 kDa).
IP Use at 1-10 µg/mg of lysate.

Target

  • Function

    Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers.
  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Contains 1 C3H1-type zinc finger.
    Contains 1 TFIIS N-terminal domain.
  • Post-translational
    modifications

    Phosphorylated on Ser-398 by PKA within the region necessary for interaction with PPP1CA.
  • Cellular localization

    Nucleus. Found in discrete nucleoplasmic bodies and within nucleoli. Associates with chromatin during interphase, excluded from condensed chromosomes during early mitosis and is reloaded onto chromosomes at the late telophase (By similarity).
  • Information by UniProt
  • Database links

  • Alternative names

    • CAT 53 antibody
    • CAT53 antibody
    • FB 19 antibody
    • FB19 antibody
    • FB19 protein antibody
    • HLA-C associated transcript 53 antibody
    • MHC class I region proline rich protein CAT53 antibody
    • MHC class I region proline-rich protein CAT53 antibody
    • p99 antibody
    • Phosphatase 1 nuclear targeting subunit antibody
    • Phosphatase nuclear targeting subunit antibody
    • PNUTS antibody
    • PP1 binding protein of 114 kDa antibody
    • PP1 nuclear targeting subunit antibody
    • PP1-binding protein of 114 kDa antibody
    • PP1R10 antibody
    • PP1RA_HUMAN antibody
    • ppp1r10 antibody
    • Protein FB19 antibody
    • Protein phosphatase 1 nuclear targeting subunit antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 10 antibody
    • Protein phosphatase 1 regulatory subunit 10 antibody
    • R111 antibody
    • Serine/threonine protein phosphatase 1 regulatory subunit 10 antibody
    • Serine/threonine-protein phosphatase 1 regulatory subunit 10 antibody
    see all

Images

  • Lanes 1-2 : Anti-PNUTS antibody (ab70248) at 1 µg/ml
    Lanes 3-4 : Anti-PNUTS antibody (ab70248) at 0.2 µg/ml

    Lanes 1 & 3 : Nuclear extracts from Hela cells at 15 µg
    Lanes 2 & 4 : Nuclear extracts from Hela cells at 45 µg

    Predicted band size: 99 kDa
    Observed band size: 115 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation/ Western Blot of PNUTS Lane 1: Another antibody which recognizes a different epitope of PNUTS. Lane 2: Another antibody which recognizes a different epitope of PNUTS. Lane 3: Another antibody which recognizes a different epitope of PNUTS. Lane 4: ab70248 at 1µg/100µg nuclear extract. Detection: Chemiluminescence with exposure times of 3 seconds.

References

ab70248 has not yet been referenced specifically in any publications.

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