Overview

  • Product name

    Anti-PNUTS antibody [EPR11706]
    See all PNUTS primary antibodies
  • Description

    Rabbit monoclonal [EPR11706] to PNUTS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PNUTS aa 550-650 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96QC0

  • Positive control

    • HeLa, HepG2 and Jurkat cell lysates; Human fetal brain lysate; Human breast and colon tissues; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as PPP1R10

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab173285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
ICC/IF 1/100 - 1/250.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Scaffold protein which mediates the formation of the PTW/PP1 phosphatase complex by providing a binding platform to each component of the complex. The PTW/PP1 phosphatase complex plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Mediates interaction of WDR82 and PPP1CA. Inhibitor of PPP1CA and PPP1CC phosphatase activities. Has inhibitory activity on PPP1CA only when phosphorylated. Binds to mRNA, single-stranded DNA (ssDNA), poly(A) and poly(G) homopolymers.
    • Tissue specificity

      Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities

      Contains 1 C3H1-type zinc finger.
      Contains 1 TFIIS N-terminal domain.
    • Post-translational
      modifications

      Phosphorylated on Ser-398 by PKA within the region necessary for interaction with PPP1CA.
    • Cellular localization

      Nucleus. Found in discrete nucleoplasmic bodies and within nucleoli. Associates with chromatin during interphase, excluded from condensed chromosomes during early mitosis and is reloaded onto chromosomes at the late telophase (By similarity).
    • Information by UniProt
    • Database links

    • Alternative names

      • CAT 53 antibody
      • CAT53 antibody
      • FB 19 antibody
      • FB19 antibody
      • FB19 protein antibody
      • HLA-C associated transcript 53 antibody
      • MHC class I region proline rich protein CAT53 antibody
      • MHC class I region proline-rich protein CAT53 antibody
      • p99 antibody
      • Phosphatase 1 nuclear targeting subunit antibody
      • Phosphatase nuclear targeting subunit antibody
      • PNUTS antibody
      • PP1 binding protein of 114 kDa antibody
      • PP1 nuclear targeting subunit antibody
      • PP1-binding protein of 114 kDa antibody
      • PP1R10 antibody
      • PP1RA_HUMAN antibody
      • ppp1r10 antibody
      • Protein FB19 antibody
      • Protein phosphatase 1 nuclear targeting subunit antibody
      • Protein phosphatase 1 regulatory (inhibitor) subunit 10 antibody
      • Protein phosphatase 1 regulatory subunit 10 antibody
      • R111 antibody
      • Serine/threonine protein phosphatase 1 regulatory subunit 10 antibody
      • Serine/threonine-protein phosphatase 1 regulatory subunit 10 antibody
      see all

    Images

    • All lanes : Anti-PNUTS antibody [EPR11706] (ab173285) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 99 kDa

    • Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PNUTS with ab173285 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PNUTS with ab173285 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunofluorescent analysis of HeLa cells labeling PNUTS with ab173285 at 1/100 dilution.

    References

    ab173285 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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