Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272633 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in early steps of centriole duplication, as well as in the later steps of centriole length control. Required for ciliogenesis.
Involvement in diseaseDefects in POC1A are the cause of short stature, onychodysplasia, facial dysmorphism, and hypotrichosis (SOFT) [MIM:614813]. A syndrome characterized by severely short long bones, peculiar facies associated with paucity of hair, and nail anomalies. Growth retardation is evident on prenatal ultrasound as early as the second trimester of pregnancy, and affected individuals reach a final stature consistent with a height age of 6 years to 8 years. Relative macrocephaly is present during early childhood but head circumference is markedly low by adulthood. Psychomotor development is normal. Facial dysmorphism includes a long, triangular face with prominent nose and small ears, and affected individuals have an unusual high-pitched voice. Clinodactyly, brachydactyly, and hypoplastic distal phalanges and fingernails are present in association with postpubertal sparse and short hair. Typical skeletal findings include short and thick long bones with mild irregular metaphyseal changes, short femoral necks, and hypoplastic pelvis and sacrum. All long bones of the hand are short, with major delay of carpal ossification and cone-shaped epiphyses. Vertebral body ossification is also delayed. Note=Cells derived from affected individuals have abnormal mitotic mechanics with multipolar spindles, in addition to clearly impaired ciliogenesis.
Sequence similaritiesBelongs to the WD repeat POC1 family.
Contains 7 WD repeats.
Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > cilium basal body. Component of both mother and daughter centrioles.
- Information by UniProt
- Pix2 antibody
- POC1 centriolar protein homolog A (Chlamydomonas) antibody
- POC1 centriolar protein homolog A antibody
All lanes : Anti-POC1A antibody (ab272633) at 0.4 µg/ml
Lane 1 : Control (vector only transfected HEK-293T lysate)
Lane 2 : POC1A over-expression lysate (mammalian HEK-293T cells)
Paraffin-embedded formalin-fixed Human rectum tissue stained for POC1A using ab272633 at 1/200 in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272633 has not yet been referenced specifically in any publications.