Recombinant Anti-POC5 antibody [EPR14000] (ab182554)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14000] to POC5
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-POC5 antibody [EPR14000]
See all POC5 primary antibodies -
Description
Rabbit monoclonal [EPR14000] to POC5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa and Jurkat cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14000 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa).
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa). |
Target
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Function
Essential for the assembly of the distal half of centrioles, required for centriole elongation. -
Sequence similarities
Belongs to the POC5 family. -
Post-translational
modificationsHyperphosphorylated during recruitment to procentrioles in G2/M phase. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome > centriole. Localized to the distal portion of centrioles. - Information by UniProt
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Database links
- Entrez Gene: 134359 Human
- SwissProt: Q8NA72 Human
- Unigene: 432726 Human
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Alternative names
- C5orf37 antibody
- Centrosomal protein POC5 antibody
- FLJ35779 antibody
see all
Images
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All lanes : Anti-POC5 antibody [EPR14000] (ab182554) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab182554 has not yet been referenced specifically in any publications.