Anti-PODN antibody (ab140180)
Key features and details
- Rabbit polyclonal to PODN
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PODN antibody -
Description
Rabbit polyclonal to PODN -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment, corresponding to amino acids 69-236 of Human PODN (BC030608).
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Positive control
- 293T cell lysate; Human fetal kidney tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:Reconstitute in 200 µl sterile water. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab140180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/2000. Predicted molecular weight: 69 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
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Function
Negatively regulates cell proliferation and cell migration. -
Tissue specificity
Kidney, heart, liver, pancreas and vascular smooth muscle cells. -
Sequence similarities
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class V subfamily.
Contains 20 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cytoplasm and Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 127435 Human
- Entrez Gene: 242608 Mouse
- Entrez Gene: 313483 Rat
- GenBank: BC030608 Human
- Omim: 608661 Human
- SwissProt: Q7Z5L7 Human
- SwissProt: Q7TQ62 Mouse
- Unigene: 586141 Human
see all -
Alternative names
- 9430070G18 antibody
- LRRGT00160 antibody
- MGC24995 antibody
see all
Images
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Western blot - Anti-PODN antibody (ab140180)Anti-PODN antibody (ab140180) at 1/1000 dilution + 293T cell lysate
Predicted band size: 69 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PODN antibody (ab140180)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fetal kidney tissue labelling PODN with ab140180 at 1/100 dilution.
Protocols
References (0)
ab140180 has not yet been referenced specifically in any publications.
