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    podoplanin--gp36-antibody-ab10274.pdf

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Anti-Podoplanin / gp36 antibody (ab10274)

  • Datasheet
Reviews (1)Q&A (3)References (4)

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Western blot - Anti-Podoplanin / gp36 antibody (ab10274)

    Key features and details

    • Rabbit polyclonal to Podoplanin / gp36
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Podoplanin / gp36 antibody [LpMab-7] (ab256561)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Podoplanin / gp36 antibody
      See all Podoplanin / gp36 primary antibodies
    • Description

      Rabbit polyclonal to Podoplanin / gp36
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant fragment (His-tag) corresponding to Human Podoplanin/ gp36 aa 1-150.
      Database link: Q86YL7

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: LEC whole cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Lyophilized:The lyophilized IgG is stable at 4°C for at least one month and for more than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six months at 2-4°C in the presence of a preservative. Reconstituted antibody can also be aliquoted and stored at –20°C to –70°C for at least 1 year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Blood
      • Other
      • Tags & Cell Markers
      • Cell Type Markers
      • Other Cell Types
      • Cardiovascular
      • Vasculature
      • Endothelium
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Podoplanin / gp36 protein (ab132033)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10274 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
    Notes
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.

    Target

    • Function

      May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
    • Tissue specificity

      Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
    • Sequence similarities

      Belongs to the podoplanin family.
    • Post-translational
      modifications

      Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
      The N-terminus is blocked.
    • Cellular localization

      Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
    • Target information above from: UniProt accession Q86YL7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10630 Human
      • Omim: 608863 Human
      • SwissProt: Q86YL7 Human
      • Unigene: 468675 Human
      • Alternative names

        • Aggrus antibody
        • Glycoprotein 36 KD antibody
        • Glycoprotein 36 antibody
        • gp 36 antibody
        • GP 38 antibody
        • GP 40 antibody
        • gp36 antibody
        • GP38 antibody
        • GP40 antibody
        • HT1A 1 antibody
        • HT1A1 antibody
        • hT1alpha 1 antibody
        • hT1alpha 2 antibody
        • hT1alpha1 antibody
        • hT1alpha2 antibody
        • Lung type I cell membrane associated glycoprotein antibody
        • Lung type I cell membrane associated glycoprotein isoform a antibody
        • Lung type I cell membrane associated glycoprotein T1A 2 antibody
        • OTS 8 antibody
        • OTS8 antibody
        • OTTHUMP00000009640 antibody
        • OTTHUMP00000044504 antibody
        • PA2.26 antibody
        • PA2.26 antigen antibody
        • Pdpn antibody
        • PDPN_HUMAN antibody
        • Podoplanin antibody
        • PSEC0003 antibody
        • PSEC0025 antibody
        • T1 alpha antibody
        • T1 ALPHA GENE antibody
        • T1-alpha antibody
        • T1A 2 antibody
        • T1A antibody
        • TI1A antibody
        • TIA 2 antibody
        • TIA2 antibody
        see all

      Images

      • Western blot - Anti-Podoplanin / gp36 antibody (ab10274)
        Western blot - Anti-Podoplanin / gp36 antibody (ab10274)

        Western Blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC). The total lysate was subjected to SDS-PAGE and subsequent Western analysis with the polyclonal antibody ab10274.

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (4)

      Publishing research using ab10274? Please let us know so that we can cite the reference in this datasheet.

      ab10274 has been referenced in 4 publications.

      • Kwak J  et al. Isolation and characterization of tumorspheres from a recurrent pineoblastoma patient: Feasibility of a patient-derived xenograft. Int J Oncol 49:569-78 (2016). PubMed: 27277549
      • Carden CP  et al. The association of PI3 kinase signaling and chemoresistance in advanced ovarian cancer. Mol Cancer Ther 11:1609-17 (2012). PubMed: 22556379
      • Kong BH  et al. Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens. Childs Nerv Syst : (2012). PubMed: 23143002
      • Link A  et al. Association of T-zone reticular networks and conduits with ectopic lymphoid tissues in mice and humans. Am J Pathol 178:1662-75 (2011). PubMed: 21435450

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Podoplanin / gp36 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (rat alveolar sections)
      Specification
      rat alveolar sections
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.2% saponin
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 08 2012

      Question

      I'd like to enquire about the gp36 Podoplanin antibody (ab10274). Where does the epitope of the antibody lie ? Is it in the extracellular or in the intracellular domain of Podoplanin ? This information would be very important for us since we are using the antibody in our lab and now want to use it for blocking the protein on its extracellular domain.

      Read More

      Abcam community

      Verified customer

      Asked on Sep 12 2012

      Answer

      Thank you for your inquiry.

      The antibody you mentioned is a polyclonal antibody against the full length protein. Polyclonal antibodies consist of a mixed population of IgGs each of which will recognize a different epitope.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

      Read More

      Abcam Scientific Support

      Answered on Sep 12 2012

      Question

      We have recently purchased antibody against gp36 (ab10274) from "Abcam Antibodies" and we want to use it on formalin or paraformaldehyde fixed tissues. On the antibody data sheet, you say that these fixatives may be modifying the epitope recognized by the antibody, so you advise to try using antigen retrieval methods to unmask the epitope. We would be very grateful if you could give us some information on the specific antigen retrieval techniques to use with this antibody. Do you have an idea whether microwave (which type of buffer, pH), enzyme digestion (which enzyme? time and concentration conditions?), or any other method should be more appropiate for this antibody?

      Read More

      Abcam community

      Verified customer

      Asked on May 03 2004

      Answer

      Thank you very much for your enquiry. Ab10274 has yet to be tested on formalin fixed, paraffin embedded tissue so I can only refer you to our general protocols which can be found on our homepage. I would suggest starting with the heat mediated pressure cooker method for antigen retrieval and a detailed protocol for this can be found in the antigen retrieval protocol section. Please do contact us again if you have any more questions.

      Read More

      Abcam Scientific Support

      Answered on May 04 2004

      Question

      DESCRIPTION OF THE PROBLEM No staining SAMPLE We have samples of skin and we know that this tissue have lymphatic vases but we have not got staining DETECTION METHOD We have use Envision method with DAB. FIXATION OF SAMPLE We have fixed our samples with formol and Bouin and we have not got staining. Would you tell me the product that you use in the fixation? ANTIGEN RETRIEVAL I have read that you teste this antibody in immunofluorescence. Do you use some of this methods?

      Read More

      Abcam community

      Verified customer

      Asked on Apr 22 2004

      Answer

      Thank you for your enquiry and your patience. We are very sorry to hear that you are having problem with this antibody We would like to confirm that this antibody has been tested for IF application. We would suggest the followings: 1. Test the detection system with another primary antibody to make sure that it works properly. Is the secondary antibody compatible with the primary antibody? Actually, DAB is not a fluorescent agent and we would recommend using either FITC or Texas Red-labelled secondary antibody for IF studies. 2. Try this antibody at 1:100 and adjust accordingly from the results obtained. 3. You may need to test different fixatives, try ice-cold (for 10 min) acetone, or ethanol or methanol. Please take a look at the following websites, you may find some useful information about the thechnique of IF and the fixation: http://www.bdbiosciences.com/pharmingen/protocols/Immunofluorescence_Microscopy.shtml http://www.biochem.emory.edu/labs/acorbe2/protocols13.html http://www.science.mcmaster.ca/biochem/faculty/andrews/lab/projects/methodsandprograms/labman/tc19.html http://www.kcl.ac.uk/depsta/biomedical/randall/methods/im-fluo.html We hope this will be of some help. But please get back to us should you have further questions or need further clarification.

      Read More

      Abcam Scientific Support

      Answered on Apr 29 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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