Recombinant
RabMAb

Recombinant Anti-Podoplanin / gp36 antibody [EPR7072] (ab128994)

Overview

  • Product name

    Anti-Podoplanin / gp36 antibody [EPR7072]
    See all Podoplanin / gp36 primary antibodies
  • Description

    Rabbit monoclonal [EPR7072] to Podoplanin / gp36
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Podoplanin/ gp36 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Human tonsil, fetal lung or 293T lysate, Human placenta tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
    • Tissue specificity

      Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
    • Sequence similarities

      Belongs to the podoplanin family.
    • Post-translational
      modifications

      Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
      The N-terminus is blocked.
    • Cellular localization

      Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
    • Information by UniProt
    • Database links

    • Alternative names

      • Aggrus antibody
      • Glycoprotein 36 KD antibody
      • Glycoprotein 36 antibody
      • gp 36 antibody
      • GP 38 antibody
      • GP 40 antibody
      • gp36 antibody
      • GP38 antibody
      • GP40 antibody
      • HT1A 1 antibody
      • HT1A1 antibody
      • hT1alpha 1 antibody
      • hT1alpha 2 antibody
      • hT1alpha1 antibody
      • hT1alpha2 antibody
      • Lung type I cell membrane associated glycoprotein antibody
      • Lung type I cell membrane associated glycoprotein isoform a antibody
      • Lung type I cell membrane associated glycoprotein T1A 2 antibody
      • OTS 8 antibody
      • OTS8 antibody
      • OTTHUMP00000009640 antibody
      • OTTHUMP00000044504 antibody
      • PA2.26 antibody
      • PA2.26 antigen antibody
      • Pdpn antibody
      • PDPN_HUMAN antibody
      • Podoplanin antibody
      • PSEC0003 antibody
      • PSEC0025 antibody
      • T1 alpha antibody
      • T1 ALPHA GENE antibody
      • T1-alpha antibody
      • T1A 2 antibody
      • T1A antibody
      • TI1A antibody
      • TIA 2 antibody
      • TIA2 antibody
      see all

    Images

    • ab128994, at a 1/250 dilution, staining Human Podoplanin / gp36 in pancreas, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • All lanes : Anti-Podoplanin / gp36 antibody [EPR7072] (ab128994) at 1/1000 dilution

      Lane 1 : Human tonsil cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Fetal lung cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 17 kDa

    • ab128994 showing positive staining in Ovarian carcinoma tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab128994 showing positive staining in Cervical carcinoma tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab128994 showing positive staining in Normal lung tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Alsafadi HN  et al. An ex vivo model to induce early fibrosis-like changes in human precision-cut lung slices. Am J Physiol Lung Cell Mol Physiol 312:L896-L902 (2017). Read more (PubMed: 28314802) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you for your telephone enquiry.

    I have contacted the laboratory for further information regarding this western blot image.

    The reason that the band is a higher MW than what is predicted by Swiss-Prot protein database site is that Swiss-Prot’s predication is only based on the number of amino acids, and does not take into account modifications.

    http://www.uniprot.org/uniprot/Q86YL7#Q86YL7

    According to this Swisprot page for this protein, it is very highly glycosylated so the actual molecular weight is likely to be much higher than the predicted. The sequence used to raise the antibody has 100% homology with isoforms 1, 3 and 4, so if they are expressed in the sample the antibody should be able to detect them. We typically only see one band in our in-house testing.

    I hope this information is helpful to you. If you have any further questions, please do not hesitate to contact me.

    Read More

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