Recombinant
RabMAb

Recombinant Anti-Podoplanin / gp36 antibody [EPR7072] - BSA and Azide free (ab235141)

Overview

  • Product name

    Anti-Podoplanin / gp36 antibody [EPR7072] - BSA and Azide free
    See all Podoplanin / gp36 primary antibodies
  • Description

    Rabbit monoclonal [EPR7072] to Podoplanin / gp36 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Podoplanin/ gp36 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human pancreas tissue.
  • General notes

    Ab235141 is the carrier-free version of ab128994. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab235141 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab235141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
    • Tissue specificity

      Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
    • Sequence similarities

      Belongs to the podoplanin family.
    • Post-translational
      modifications

      Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
      The N-terminus is blocked.
    • Cellular localization

      Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
    • Information by UniProt
    • Database links

    • Alternative names

      • Aggrus antibody
      • Glycoprotein 36 KD antibody
      • Glycoprotein 36 antibody
      • gp 36 antibody
      • GP 38 antibody
      • GP 40 antibody
      • gp36 antibody
      • GP38 antibody
      • GP40 antibody
      • HT1A 1 antibody
      • HT1A1 antibody
      • hT1alpha 1 antibody
      • hT1alpha 2 antibody
      • hT1alpha1 antibody
      • hT1alpha2 antibody
      • Lung type I cell membrane associated glycoprotein antibody
      • Lung type I cell membrane associated glycoprotein isoform a antibody
      • Lung type I cell membrane associated glycoprotein T1A 2 antibody
      • OTS 8 antibody
      • OTS8 antibody
      • OTTHUMP00000009640 antibody
      • OTTHUMP00000044504 antibody
      • PA2.26 antibody
      • PA2.26 antigen antibody
      • Pdpn antibody
      • PDPN_HUMAN antibody
      • Podoplanin antibody
      • PSEC0003 antibody
      • PSEC0025 antibody
      • T1 alpha antibody
      • T1 ALPHA GENE antibody
      • T1-alpha antibody
      • T1A 2 antibody
      • T1A antibody
      • TI1A antibody
      • TIA 2 antibody
      • TIA2 antibody
      see all

    Images

    • ab128994 showing positive staining in Ovarian carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128994).

    • ab128994 showing positive staining in Cervical carcinoma tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128994).

    • ab128994 showing positive staining in Normal lung tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128994).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128994).

    • ab128994, at a 1/250 dilution, staining human Podoplanin / gp36 in pancreas, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing tissue culture supernatant, PBS, BSA, glycerol and sodium azide (ab128994).

    References

    ab235141 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab235141.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up