Recombinant Anti-Podoplanin antibody [EPR7073] (ab131216)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7073] to Podoplanin
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Podoplanin antibody [EPR7073]
See all Podoplanin primary antibodies -
Description
Rabbit monoclonal [EPR7073] to Podoplanin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human tonsil, uterus cancer, placenta tissue, HT 1080 and fetal lung lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7073 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 36-45 kDa (predicted molecular weight: 16 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 36-45 kDa (predicted molecular weight: 16 kDa). |
Target
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Function
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. -
Tissue specificity
Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. -
Sequence similarities
Belongs to the podoplanin family. -
Post-translational
modificationsExtensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
The N-terminus is blocked. -
Cellular localization
Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. - Information by UniProt
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Database links
- Entrez Gene: 10630 Human
- Entrez Gene: 14726 Mouse
- Entrez Gene: 54320 Rat
- Omim: 608863 Human
- SwissProt: Q86YL7 Human
- SwissProt: Q62011 Mouse
- SwissProt: Q64294 Rat
- Unigene: 468675 Human
see all -
Alternative names
- Aggrus antibody
- D2-40 antibody
- Glycoprotein 36 KD antibody
see all
Images
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All lanes : Anti-Podoplanin antibody [EPR7073] (ab131216) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human tonsil lysate
Lane 3 : Human uterus cancer lysate
Lane 4 : Human placenta lysate
Lane 5 : HT 1080 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab131216 has been referenced in 2 publications.
- Fang Q et al. pre-B cell colony enhancing factor negatively regulates Na+ and fluid transport in lung epithelial cells. Am J Transl Res 10:2047-2054 (2018). PubMed: 30093942
- Xu Y et al. Polypeptide N-Acetylgalactosaminyltransferase 13 Contributes to Neurogenesis via Stabilizing the Mucin-type O-Glycoprotein Podoplanin. J Biol Chem 291:23477-23488 (2016). PubMed: 27629416