Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7073] to Podoplanin / gp36
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Podoplanin / gp36 antibody [EPR7073]
See all Podoplanin / gp36 primary antibodies
DescriptionRabbit monoclonal [EPR7073] to Podoplanin / gp36
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Podoplanin/ gp36 aa 150-250 (C terminal). The exact sequence is proprietary.
- Human tonsil, uterus cancer, placenta tissue, HT 1080 and fetal lung lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 3.30 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 36-45 kDa (predicted molecular weight: 16 kDa).|
FunctionMay be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
Tissue specificityHighly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
Sequence similaritiesBelongs to the podoplanin family.
modificationsExtensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
The N-terminus is blocked.
Cellular localizationMembrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
- Information by UniProt
- Aggrus antibody
- Glycoprotein 36 KD antibody
- Glycoprotein 36 antibody
All lanes : Anti-Podoplanin / gp36 antibody [EPR7073] (ab131216) at 1/1000 dilution
Lane 1 : Human fetal lung lysate
Lane 2 : Human tonsil lysate
Lane 3 : Human uterus cancer lysate
Lane 4 : Human placenta lysate
Lane 5 : HT 1080 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab131216 has been referenced in 2 publications.
- Fang Q et al. pre-B cell colony enhancing factor negatively regulates Na+ and fluid transport in lung epithelial cells. Am J Transl Res 10:2047-2054 (2018). PubMed: 30093942
- Xu Y et al. Polypeptide N-Acetylgalactosaminyltransferase 13 Contributes to Neurogenesis via Stabilizing the Mucin-type O-Glycoprotein Podoplanin. J Biol Chem 291:23477-23488 (2016). PubMed: 27629416