Anti-Podoplanin antibody [RTD4E10] - BSA and Azide free (ab11936)
Key features and details
- Syrian hamster monoclonal [RTD4E10] to Podoplanin - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-Podoplanin antibody [RTD4E10] - BSA and Azide free
See all Podoplanin primary antibodies -
Description
Syrian hamster monoclonal [RTD4E10] to Podoplanin - BSA and Azide free -
Host species
Syrian hamster -
Specificity
The monoclonal antibody will detect podoplanin on the surface of mouse lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry. This antibody is designed to detect podoplanin, which is also referred to as gp38 (mouse and rat) and gp36 (human). -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse
Does not react with: Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Mouse normal skin FFPE tissue sections (IHC-P)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
RTD4E10 -
Isotype
IgG1 -
Research areas
Associated products
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Conjugation kits
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (6) |
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. -
Tissue specificity
Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. -
Sequence similarities
Belongs to the podoplanin family. -
Post-translational
modificationsExtensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
The N-terminus is blocked. -
Cellular localization
Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. - Information by UniProt
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Database links
- Entrez Gene: 14726 Mouse
- SwissProt: Q62011 Mouse
- Unigene: 2976 Mouse
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Alternative names
- Aggrus antibody
- D2-40 antibody
- Glycoprotein 36 KD antibody
see all
Images
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IHC image of Podoplanin staining in a section of formalin-fixed paraffin-embedded normal mouse skin. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab11936 at 1/250. The secondary antibody (shown in green) was ab180063 at 1/500 (Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) and counterstained using ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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ab11936 staining Podoplanin in murine skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and an enzymatic antigen retrieval step was performed using Protein K. Samples were then blocked with 5% serum for 30 minutes at room temperature and then incubated with ab11936 at a 1/400 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated goat anti-hamster IgG used at a 1/500 dilution.
Datasheets and documents
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Datasheet download
References (81)
ab11936 has been referenced in 81 publications.
- Sastourné-Arrey Q et al. Adipose tissue is a source of regenerative cells that augment the repair of skeletal muscle after injury. Nat Commun 14:80 (2023). PubMed: 36604419
- Fischer A et al. Neutrophils direct preexisting matrix to initiate repair in damaged tissues. Nat Immunol 23:518-531 (2022). PubMed: 35354953
- Fleig S et al. Loss of vascular endothelial notch signaling promotes spontaneous formation of tertiary lymphoid structures. Nat Commun 13:2022 (2022). PubMed: 35440634
- Ciccimarra R et al. The normal and fibrotic mouse lung classified by spatial proteomic analysis. Sci Rep 12:8742 (2022). PubMed: 35610327
- Kataru RP et al. Structural and Functional Changes in Aged Skin Lymphatic Vessels. Front Aging 3:864860 (2022). PubMed: 35821848