• Product name
    Anti-Podoplanin / gp36 antibody [18H5]
    See all Podoplanin / gp36 primary antibodies
  • Description
    Mouse monoclonal [18H5] to Podoplanin / gp36
  • Host species
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Podoplanin/ gp36. MDCK cells stably expressing Podoplanin/ gp36 protein.
    Database link: Q86YL7

  • Positive control
    • In Western blot, this antibody gave a positive signal in human placenta and skeletal muscle tissue lysates.



Our Abpromise guarantee covers the use of ab10288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 6 - 30 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 24 kDa).
Flow Cyt Use 1µl for 106 cells.

(methanol fixed cells)





ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IHC-Fr Use at an assay dependent concentration. PubMed: 18987360


  • Function
    May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
  • Tissue specificity
    Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
  • Sequence similarities
    Belongs to the podoplanin family.
  • Post-translational
    Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
    The N-terminus is blocked.
  • Cellular localization
    Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aggrus antibody
    • Glycoprotein 36 KD antibody
    • Glycoprotein 36 antibody
    • gp 36 antibody
    • GP 38 antibody
    • GP 40 antibody
    • gp36 antibody
    • GP38 antibody
    • GP40 antibody
    • HT1A 1 antibody
    • HT1A1 antibody
    • hT1alpha 1 antibody
    • hT1alpha 2 antibody
    • hT1alpha1 antibody
    • hT1alpha2 antibody
    • Lung type I cell membrane associated glycoprotein antibody
    • Lung type I cell membrane associated glycoprotein isoform a antibody
    • Lung type I cell membrane associated glycoprotein T1A 2 antibody
    • OTS 8 antibody
    • OTS8 antibody
    • OTTHUMP00000009640 antibody
    • OTTHUMP00000044504 antibody
    • PA2.26 antibody
    • PA2.26 antigen antibody
    • Pdpn antibody
    • PDPN_HUMAN antibody
    • Podoplanin antibody
    • PSEC0003 antibody
    • PSEC0025 antibody
    • T1 alpha antibody
    • T1 ALPHA GENE antibody
    • T1-alpha antibody
    • T1A 2 antibody
    • T1A antibody
    • TI1A antibody
    • TIA 2 antibody
    • TIA2 antibody
    see all


  • ab10288 at 1/500 dilution staining human thymus specimen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat induced epitope retrieval step was carried out. The tisse was then blocked and incubated with the antibody. An HRP conjugated rabbit antibody was used as the secondary.

    See Abreview

  • ab10288 at a 1/500 dilution staining human dermal lymphatic endothelial by Immunocytochemistry. The cells were grown in coverslips and fixed in formalin prior to permeabilization with tritonX-100. The cells were incubated with the primary antibody at 1/500 for 1 hour and then bound antibody was detected using a biotinylated goat anti-mouse antibody. Texas red-streptavidin was used for development.

    This image is courtesy of an Abreview submitted by Maria Navarro.

    See Abreview

  • All lanes : Anti-Podoplanin / gp36 antibody [18H5] (ab10288) at 5 µg/ml

    Lane 1 : Human placenta tissue lysate - total protein (ab29745)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?

    Exposure time: 20 minutes

    Podoplanin protein is extensively glycosylated. This might explain the observation of a band at a higher molecular weight than predicted.
  • Overlay histogram showing HCT116 cells stained with ab10288 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10288, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a slightly decreased signal in HCT116 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Western blot analysis of Podoplanin expression in human lymphatic endothelial cells (LEC) - lane 1, and HUVECs - lane 2. Total lysate of both cell types were subjected to SDS-PAGE and subsequent western blot analysis with ab10288. The antibody recognizes a protein of about 36 kDa in total lysate from LECs but not from HUVEC.

  • Immunocytochemistry/Immunofluorescence analysis of human dermal lymphatic endothelial cells (HDLEC) labelling Podoplanin / gp36 with ab10288. An Alexa Fluor® 488-conjugated goat anti-mouse was used as the secondary antibody. Nuclei counterstained with DAPI.


This product has been referenced in:
  • Zhao X  et al. Plasma soluble podoplanin is a novel marker for the diagnosis of tumor occurrence and metastasis. Cancer Sci 109:403-411 (2018). Read more (PubMed: 29266546) »
  • Nikitakis NG  et al. DSPP-MMP20 gene silencing downregulates cancer stem cell markers in human oral cancer cells. Cell Mol Biol Lett 23:30 (2018). Read more (PubMed: 30002682) »
See all 17 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris Gel)
Human Cell lysate - whole cell (SW1353 human chondrosacroma cell line)
SW1353 human chondrosacroma cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 14 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Protein-Block Serum Free (DakoCytomation) as blocking agent as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Antigen retrieval step
Sheep Tissue sections (Sheep tibia bone)
Sheep tibia bone
10% neutral-buffered formalin

Abcam user community

Verified customer

Submitted Jun 18 2013


Thank you for your recent telephone enquiry.

As discussed on the telephone, far as we are aware, ab10288 Podoplanin / gp36 antibody [18H5] has never been tested with samples from mouse. All tested species cross-reactivity information is stated on our datasheets, and these are updated as soon as any new information is brought to our attention.

I have reviewed the alignment of the immunogen for this antibody with the mouse protein, and regrettably this is low at 39%. Therefore, we would not expect this antibody to detect in mouse samples. I'm sorry this is not better news for you.

I can recommend that ab11936 Anti-Podoplanin / gp36 antibodywould be amore suitable option as it is already tested in mouse. You will need to ensure it is tested in the application you are using so it is covered by the guarantee for your experiments.


If your chosen application is not listed as tested on the datasheet, please do not hesitate to let me know before you purchase, as you may be eligible for our testing discount program.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:


As ab11936 is a hamster antibody, we have one anti hamster secondary antibody, which I hope will be helpful. Please check the datasheet for further information:

https://www.abcam.com/index.html?datasheet=11936 (or use the following: https://www.abcam.com/index.html?datasheet=11936).

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (thymus specimen)
thymus specimen
Antigen retrieval step
Heat mediated
Blocking step
protein block as blocking agent for 5 minute(s) · Concentration: 0,015 mol/

Dr. Anca Cimpean

Verified customer

Submitted Feb 02 2007


Thank you for your enquiry. The recommended method for this antibody is heat-mediated antigen retrieval with citrate buffer, pH 6.0. I have updated the datasheet with this information. I hope this helps. Please do not hesitate to contact us if you need anything further.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Human Cell (dermal lymphatic endothelial)
dermal lymphatic endothelial
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% goat

Dr. Maria Navarro

Verified customer

Submitted May 03 2006


Thank you for your enquiry. I can confirm for you that this antibody, ab10288, is indeed a mouse monoclonal antibody (clone number 18H5). The statement in the relevance section, "Produced from sera of rabbits immunised with the recombinant ectodomain of Podoplanin(gp36)" is a mistake and I have removed it. The statement refers to the rabbit polyclonal gp36/podoplanin antibody, ab10274. I apologize for the confusion. If you have any additional questions please contact us again.

Read More


Thank you for your patience. We would like to confirm that this antibody has been tested on paraffin embedded section.

Read More


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