Overview

  • Product name
    Anti-Podoplanin / gp36 antibody
    See all Podoplanin / gp36 primary antibodies
  • Description
    Rabbit polyclonal to Podoplanin / gp36
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

Properties

  • Form
    Lyophilised:The lyophilized IgG is stable at 4°C for at least one month and for more than a year when kept at –20°C. When reconstituted in sterile water to a concentration of >0.5 mg/ml the antibody is stable for at least six months at 2-4°C in the presence of a preservative. Reconstituted antibody can also be aliquoted and stored at –20°C to –70°C for at least 1 year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100.
WB 1/5000. Predicted molecular weight: 24 kDa.
ICC/IF 1/400.

Target

  • Function
    May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels.
  • Tissue specificity
    Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
  • Sequence similarities
    Belongs to the podoplanin family.
  • Post-translational
    modifications
    Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
    The N-terminus is blocked.
  • Cellular localization
    Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aggrus antibody
    • Glycoprotein 36 KD antibody
    • Glycoprotein 36 antibody
    • gp 36 antibody
    • GP 38 antibody
    • GP 40 antibody
    • gp36 antibody
    • GP38 antibody
    • GP40 antibody
    • HT1A 1 antibody
    • HT1A1 antibody
    • hT1alpha 1 antibody
    • hT1alpha 2 antibody
    • hT1alpha1 antibody
    • hT1alpha2 antibody
    • Lung type I cell membrane associated glycoprotein antibody
    • Lung type I cell membrane associated glycoprotein isoform a antibody
    • Lung type I cell membrane associated glycoprotein T1A 2 antibody
    • OTS 8 antibody
    • OTS8 antibody
    • OTTHUMP00000009640 antibody
    • OTTHUMP00000044504 antibody
    • PA2.26 antibody
    • PA2.26 antigen antibody
    • Pdpn antibody
    • PDPN_HUMAN antibody
    • Podoplanin antibody
    • PSEC0003 antibody
    • PSEC0025 antibody
    • T1 alpha antibody
    • T1 ALPHA GENE antibody
    • T1-alpha antibody
    • T1A 2 antibody
    • T1A antibody
    • TI1A antibody
    • TIA 2 antibody
    • TIA2 antibody
    see all

References

This product has been referenced in:
  • Carden CP  et al. The association of PI3 kinase signaling and chemoresistance in advanced ovarian cancer. Mol Cancer Ther 11:1609-17 (2012). Read more (PubMed: 22556379) »
  • Kong BH  et al. Isolation of glioma cancer stem cells in relation to histological grades in glioma specimens. Childs Nerv Syst : (2012). Read more (PubMed: 23143002) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (rat alveolar sections)
Specification
rat alveolar sections
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% saponin
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 08 2012

Answer

Thank you for your inquiry.

The antibody you mentioned is a polyclonal antibody against the full length protein. Polyclonal antibodies consist of a mixed population of IgGs each of which will recognize a different epitope.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

Read More

Answer

Thank you very much for your enquiry. Ab10274 has yet to be tested on formalin fixed, paraffin embedded tissue so I can only refer you to our general protocols which can be found on our homepage. I would suggest starting with the heat mediated pressure cooker method for antigen retrieval and a detailed protocol for this can be found in the antigen retrieval protocol section. Please do contact us again if you have any more questions.

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Answer

Thank you for your enquiry and your patience. We are very sorry to hear that you are having problem with this antibody We would like to confirm that this antibody has been tested for IF application. We would suggest the followings: 1. Test the detection system with another primary antibody to make sure that it works properly. Is the secondary antibody compatible with the primary antibody? Actually, DAB is not a fluorescent agent and we would recommend using either FITC or Texas Red-labelled secondary antibody for IF studies. 2. Try this antibody at 1:100 and adjust accordingly from the results obtained. 3. You may need to test different fixatives, try ice-cold (for 10 min) acetone, or ethanol or methanol. Please take a look at the following websites, you may find some useful information about the thechnique of IF and the fixation: http://www.bdbiosciences.com/pharmingen/protocols/Immunofluorescence_Microscopy.shtml http://www.biochem.emory.edu/labs/acorbe2/protocols13.html http://www.science.mcmaster.ca/biochem/faculty/andrews/lab/projects/methodsandprograms/labman/tc19.html http://www.kcl.ac.uk/depsta/biomedical/randall/methods/im-fluo.html We hope this will be of some help. But please get back to us should you have further questions or need further clarification.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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