Key features and details
- Rabbit polyclonal to PODXL - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human, Monkey
- Isotype: IgG
Product nameAnti-PODXL antibody - N-terminal
See all PODXL primary antibodies
DescriptionRabbit polyclonal to PODXL - N-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human, Monkey
Synthetic peptide within Human PODXL (N terminal). The exact sequence is proprietary. NP_001018121.1.
Database link: O00592
- Rat kidney lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 30% Glycerol
Proprietary buffer: Tris/Glycine/HCL buffer pH7.4-7.8 with stabilizing proteins (0.5%).
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab205350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 27222353|
|WB||1/500. Predicted molecular weight: 59 kDa.|
FunctionInvolved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
Tissue specificityGlomerular epithelium cell (podocyte).
Sequence similaritiesBelongs to the podocalyxin family.
DomainBoth the O-glycan-rich domain of the extracellular domain and the C-terminus PDZ-binding motif (DTHL) in the cytoplasmic tail harbor an apical sorting signal. The cytoplasmic domain is necessary for the apical membrane targeting and renal tubulogenesis. The cytoplasmic C-terminus PDZ-binding motif (DTHL) is essential for interaction with SLC9A3R1 and for targeting SLC9A3R1 to the apical cell membrane. The extracellular domain is necessary for microvillus formation (By similarity). The large highly anionic extracellular domain allows to maintain open filtration pathways between neighboring podocyte foot processes.
modificationsN- and O-linked glycosylated. Sialoglycoprotein.
Cellular localizationApical cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Membrane raft. Membrane. In single attached epithelial cells is restricted to a preapical pole on the free plasma membrane whereas other apical and basolateral proteins are not yet polarized. Colocalizes with SLC9A3R2 at the apical plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans-Golgi network (transiently) and at the apical plasma membrane. Its association with the membrane raft is transient. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apical cell surface where microvilli form. Colocalizes with EZR and SLC9A3R2 at the apical cell membrane of glomerular epithelium cells (By similarity). Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis (By similarity).
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- GCTM-2 antigen antibody
- Gp2 antibody
- Gp200 antibody
Anti-PODXL antibody - N-terminal (ab205350) at 1/500 dilution + rat kidney lysate
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
ab205350 has been referenced in 3 publications.
- Zhi Q et al. Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein. Cancer Sci 110:118-134 (2019). PubMed: 30407695
- Li TS et al. Magnesium isoglycyrrhizinate ameliorates fructose-induced podocyte apoptosis through downregulation of miR-193a to increase WT1. Biochem Pharmacol 166:139-152 (2019). PubMed: 31085161
- Zhao Q et al. Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction. Mol Med Rep 14:681-8 (2016). IHC-P ; Rat . PubMed: 27222353