Anti-Pofut1 antibody (ab154051)
Key features and details
- Rabbit polyclonal to Pofut1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Pofut1 antibody
See all Pofut1 primary antibodies -
Description
Rabbit polyclonal to Pofut1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-210 of Human Pofut1 (Uniprot ID: Q9H488).
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Positive control
- HCT116, Mouse liver and HUVEC whole cell lysates; HCT116 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling. -
Tissue specificity
Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 68 family. -
Cellular localization
Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 360194 Cow
- Entrez Gene: 609881 Dog
- Entrez Gene: 23509 Human
- Entrez Gene: 140484 Mouse
- Entrez Gene: 397627 Pig
- Entrez Gene: 311551 Rat
- Omim: 607491 Human
- SwissProt: Q9H488 Human
see all -
Alternative names
- FUT 12 antibody
- FUT12 antibody
- GDP fucose protein O fucosyltransferase 1 antibody
see all
Images
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Anti-Pofut1 antibody (ab154051) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 44 kDa
12% SDS PAGE -
Anti-Pofut1 antibody (ab154051) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 44 kDa
10% SDS PAGE -
Anti-Pofut1 antibody (ab154051) at 1/5000 dilution + HUVEC whole cell lysate at 15 µg
Predicted band size: 44 kDa
12% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HCT116 cells, labeling Pofut1 with ab154051 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154051 has been referenced in 1 publication.
- McMillan BJ et al. Structure of human POFUT1, its requirement in ligand-independent oncogenic Notch signaling, and functional effects of Dowling-Degos mutations. Glycobiology 27:777-786 (2017). PubMed: 28334865