Anti-Pofut1 antibody (ab240479)
Key features and details
- Goat polyclonal to Pofut1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Pofut1 antibody
See all Pofut1 primary antibodies -
Description
Goat polyclonal to Pofut1 -
Host species
Goat -
Specificity
This antibody is expected to recognize both reported isoforms (NP_056167.1; NP_758436.1). -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Human Pofut1 aa 50-150 (internal sequence) (Cysteine residue). (NP_056167.1; NP_758436.1)
Database link: Q9H488 -
Positive control
- WB: Pofut1 with myc and DYKDDDDK-tag transfected HEK-293 cell lysate. IHC-P: Human cerebral cortex tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 44 kDa.
1 hour primary incubation is recommended for this product. |
|
IHC-P |
Use a concentration of 2 - 4 µg/ml.
Steamed antigen retrieval with citrate buffer pH 6. |
Notes |
---|
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 44 kDa. 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 2 - 4 µg/ml. Steamed antigen retrieval with citrate buffer pH 6. |
Target
-
Function
Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling. -
Tissue specificity
Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Pathway
Protein modification; protein glycosylation. -
Sequence similarities
Belongs to the glycosyltransferase 68 family. -
Cellular localization
Endoplasmic reticulum. - Information by UniProt
-
Database links
- Entrez Gene: 449504 Chimpanzee
- Entrez Gene: 23509 Human
- Entrez Gene: 140484 Mouse
- Entrez Gene: 311551 Rat
- Omim: 607491 Human
- SwissProt: Q6EV69 Chimpanzee
- SwissProt: P83337 Chinese hamster
- SwissProt: Q9H488 Human
see all -
Alternative names
- FUT 12 antibody
- FUT12 antibody
- GDP fucose protein O fucosyltransferase 1 antibody
see all
Images
-
Lanes 1-2 : Anti-Pofut1 antibody (ab240479) at 1 µg/ml
Lane 3 : Anti-Myc tag at 1/1000 dilution
Lane 1 : Pofut1 with myc and DYKDDDDK tag transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate in RIPA buffer
Lane 2 : Untransfected HE-293 cell lysate in RIPA buffer
Lane 3 : Pofut1 with myc and DYKDDDDK-tag transfected HEK-293 cell lysate in RIPA buffer
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 44 kDaPrimary incubation for 1 hour.
-
Paraffin-embedded human cerebral cortex tissue stained for Pofut1 using ab240479 at 3 μg/ml in immunohistochemical analysis. HRP staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab240479 has not yet been referenced specifically in any publications.