Overview

  • Product name

  • Description

    Goat polyclonal to Pofut1
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognize both reported isoforms (NP_056167.1; NP_758436.1).
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chimpanzee, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human Pofut1 aa 81-95 (internal sequence) (Cysteine residue). (NP_056167.1; NP_758436.1)
    Sequence:

    C-TNLHVSYQKYFKLEP


    Database link: Q9H488

  • Positive control

    • WB: Pofut1 with myc and DYKDDDDK-tag transfected HEK-293 cell lysate. IHC-P: Human cerebral cortex tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 44 kDa.
IHC-P Use a concentration of 2 - 4 µg/ml.

Steamed antigen retrieval with citrate buffer pH 6.

Target

  • Function

    Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling.
  • Tissue specificity

    Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Belongs to the glycosyltransferase 68 family.
  • Cellular localization

    Endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • FUT 12 antibody
    • FUT12 antibody
    • GDP fucose protein O fucosyltransferase 1 antibody
    • GDP-fucose protein O-fucosyltransferase 1 antibody
    • O Fuc T antibody
    • o fucosyltransferase protein antibody
    • O FucT 1 antibody
    • O FucT antibody
    • O FucT1 antibody
    • O FUT antibody
    • O-FucT-1 antibody
    • OFUT 1 antibody
    • OFUT antibody
    • OFUT1 antibody
    • OFUT1_HUMAN antibody
    • Peptide O fucosyltransferase1 antibody
    • Peptide-O-fucosyltransferase 1 antibody
    • Pofut 1 antibody
    • POFUT1 antibody
    • Protein O fucosyltransferase1 antibody
    see all

Images

  • Lanes 1-2 : Anti-Pofut1 antibody (ab240479) at 1 µg/ml
    Lane 3 : Anti-Myc tag at 1/1000 dilution

    Lane 1 : Pofut1 with myc and DYKDDDDK tag transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate in RIPA buffer
    Lane 2 : Untransfected HE-293 cell lysate in RIPA buffer
    Lane 3 : Pofut1 with myc and DYKDDDDK-tag transfected HEK-293 cell lysate in RIPA buffer

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 44 kDa



    Primary incubation for 1 hour.

  • Paraffin-embedded human cerebral cortex tissue stained for Pofut1 using ab240479 at 3 μg/ml in immunohistochemical analysis. HRP staining.

References

ab240479 has not yet been referenced specifically in any publications.

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