Key features and details
- Goat polyclonal to Pofut1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Pofut1 antibody
See all Pofut1 primary antibodies
DescriptionGoat polyclonal to Pofut1
SpecificityThis antibody is expected to recognize both reported isoforms (NP_056167.1; NP_758436.1).
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee, Chinese hamster
- WB: Pofut1 with myc and DYKDDDDK-tag transfected HEK-293 cell lysate. IHC-P: Human cerebral cortex tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab240479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 44 kDa.
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 2 - 4 µg/ml.
Steamed antigen retrieval with citrate buffer pH 6.
FunctionCatalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling.
Tissue specificityHighly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 68 family.
Cellular localizationEndoplasmic reticulum.
- Information by UniProt
- FUT 12 antibody
- FUT12 antibody
- GDP fucose protein O fucosyltransferase 1 antibody
Lanes 1-2 : Anti-Pofut1 antibody (ab240479) at 1 µg/ml
Lane 3 : Anti-Myc tag at 1/1000 dilution
Lane 1 : Pofut1 with myc and DYKDDDDK tag transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate in RIPA buffer
Lane 2 : Untransfected HE-293 cell lysate in RIPA buffer
Lane 3 : Pofut1 with myc and DYKDDDDK-tag transfected HEK-293 cell lysate in RIPA buffer
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 44 kDa
Primary incubation for 1 hour.
Paraffin-embedded human cerebral cortex tissue stained for Pofut1 using ab240479 at 3 μg/ml in immunohistochemical analysis. HRP staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240479 has not yet been referenced specifically in any publications.