Anti-Pofut1 antibody (ab74302)

Overview

  • Product name
  • Description
    Rabbit polyclonal to Pofut1
  • Host species
    Rabbit
  • Specificity
    ab74302 detects endogenous levels of total Pofut1 protein.
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human Pofat1.

  • Positive control
    • WB: Extracts from HeLa, Jurkat and HepG2 cells. IHC-P: Human lung carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100.

Target

  • Function
    Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling.
  • Tissue specificity
    Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 68 family.
  • Cellular localization
    Endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • FUT 12 antibody
    • FUT12 antibody
    • GDP fucose protein O fucosyltransferase 1 antibody
    • GDP-fucose protein O-fucosyltransferase 1 antibody
    • O Fuc T antibody
    • o fucosyltransferase protein antibody
    • O FucT 1 antibody
    • O FucT antibody
    • O FucT1 antibody
    • O FUT antibody
    • O-FucT-1 antibody
    • OFUT 1 antibody
    • OFUT antibody
    • OFUT1 antibody
    • OFUT1_HUMAN antibody
    • Peptide O fucosyltransferase1 antibody
    • Peptide-O-fucosyltransferase 1 antibody
    • Pofut 1 antibody
    • POFUT1 antibody
    • Protein O fucosyltransferase1 antibody
    see all

Images

  • ab74302, at a 1/50 dilution, staining Pofut1 in paraffin embedded human lung carcinoma tissue in the absence (left image) and presence (right image) of the immunising peptide.
  • All lanes : Anti-Pofut1 antibody (ab74302) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from HepG2 cells
    Lane 4 : extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 44 kDa
    Observed band size: 44 kDa
    Additional bands at: 117 kDa, 22 kDa, 28 kDa, 30 kDa. We are unsure as to the identity of these extra bands.

References

ab74302 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Mar 22 2013

As for ab74302,I have received information that we do not have immunogen peptide available for sale in that case.

I apologize for any inconvenience.

Please do not hesitate to contact us if you need any more advice or information in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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