Key features and details
- Rabbit polyclonal to Pofut1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Pofut1 antibody
See all Pofut1 primary antibodies
DescriptionRabbit polyclonal to Pofut1
Specificityab74302 detects endogenous levels of total Pofut1 protein.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Synthetic peptide derived from an internal region of human Pofat1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab74302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 44 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionCatalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling.
Tissue specificityHighly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 68 family.
Cellular localizationEndoplasmic reticulum.
- Information by UniProt
- FUT 12 antibody
- FUT12 antibody
- GDP fucose protein O fucosyltransferase 1 antibody
ab74302, at a 1/50 dilution, staining Pofut1 in paraffin embedded human lung carcinoma tissue in the absence (left image) and presence (right image) of the immunising peptide.
All lanes : Anti-Pofut1 antibody (ab74302) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from Jurkat cells
Lane 3 : extracts from HepG2 cells
Lane 4 : extracts from Jurkat cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 117 kDa, 22 kDa, 28 kDa, 30 kDa. We are unsure as to the identity of these extra bands.
ab74302 has been referenced in 1 publication.
- Chabanais J et al. POFUT1 as a Promising Novel Biomarker of Colorectal Cancer. Cancers (Basel) 10:N/A (2018). PubMed: 30380753