Anti-Pofut1 antibody (ab74302)

Reviews (1)Q&A (1)References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pofut1 antibody (ab74302)
  • Western blot - Anti-Pofut1 antibody (ab74302)

Key features and details

  • Rabbit polyclonal to Pofut1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Pofut1 antibody
    See all Pofut1 primary antibodies

  • Description

    Rabbit polyclonal to Pofut1

  • Host species

    Rabbit

  • Specificity

    ab74302 detects endogenous levels of total Pofut1 protein.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide derived from an internal region of human Pofat1.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab74302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
IHC-P
1/50 - 1/100.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
IHC-P
1/50 - 1/100.

Target

  • Function

    Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in EGF domains. Plays a crucial role in Notch signaling.

  • Tissue specificity

    Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

  • Pathway

    Protein modification; protein glycosylation.

  • Sequence similarities

    Belongs to the glycosyltransferase 68 family.

  • Cellular localization

    Endoplasmic reticulum.
  • Information by UniProt

  • Database links

    • Alternative names

      • FUT 12 antibody
      • FUT12 antibody
      • GDP fucose protein O fucosyltransferase 1 antibody
      • GDP-fucose protein O-fucosyltransferase 1 antibody
      • O Fuc T antibody
      • o fucosyltransferase protein antibody
      • O FucT 1 antibody
      • O FucT antibody
      • O FucT1 antibody
      • O FUT antibody
      • O-FucT-1 antibody
      • OFUT 1 antibody
      • OFUT antibody
      • OFUT1 antibody
      • OFUT1_HUMAN antibody
      • Peptide O fucosyltransferase1 antibody
      • Peptide-O-fucosyltransferase 1 antibody
      • Pofut 1 antibody
      • POFUT1 antibody
      • Protein O fucosyltransferase1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pofut1 antibody (ab74302)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pofut1 antibody (ab74302)
      ab74302, at a 1/50 dilution, staining Pofut1 in paraffin embedded human lung carcinoma tissue in the absence (left image) and presence (right image) of the immunising peptide.
    • Western blot - Anti-Pofut1 antibody (ab74302)
      Western blot - Anti-Pofut1 antibody (ab74302)
      All lanes : Anti-Pofut1 antibody (ab74302) at 1/500 dilution

      Lane 1 : extracts from HeLa cells
      Lane 2 : extracts from Jurkat cells
      Lane 3 : extracts from HepG2 cells
      Lane 4 : extracts from Jurkat cells with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 44 kDa
      Observed band size: 44 kDa
      Additional bands at: 117 kDa, 22 kDa, 28 kDa, 30 kDa. We are unsure as to the identity of these extra bands.

    References (1)

    Publishing research using ab74302? Please let us know so that we can cite the reference in this datasheet.

    ab74302 has been referenced in 1 publication.

    • Chabanais J  et al. POFUT1 as a Promising Novel Biomarker of Colorectal Cancer. Cancers (Basel) 10:N/A (2018). PubMed: 30380753

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a question

    Question

    ab74302, ab102835: is the immunogen peptide available for sale?

    Read More
    Answer

    As for ab74302,I have received information that we do not have immunogen peptide available for sale in that case.

    I apologize for any inconvenience.

    Please do not hesitate to contact us if you need any more advice or information in this regard.

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