Anti-POGZ antibody (ab171934)
Key features and details
- Rabbit polyclonal to POGZ
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-POGZ antibody
See all POGZ primary antibodies -
Description
Rabbit polyclonal to POGZ -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human POGZ aa 1-562 (N terminal).
Sequence:MADTDLFMECEEEELEPWQKISDVIEDSVVEDYNSVDKTTTVSVSQQPVS APVPIAAHASVAGHLSTSTTVSSSGAQNSDSTKKTLVTLIANNNAGNPLV QQGGQPLILTQNPAPGLGTMVTQPVLRPVQVMQNANHVTSSPVASQPIFI TTQGFPVRNVRPVQNAMNQVGIVLNVQQGQTVRPITLVPAPGTQFVKPTV GVPQVFSQMTPVRPGSTMPVRPTTNTFTTVIPATLTIRSTVPQSQSQQTK STPSTSTTPTATQPTSLGQLAVQSPGQSNQTTNPKLAPSFPSPPAVSIAS FVTVKRPGVTGENSNEVAKLVNTLNTIPSLGQSPGPVVVSNNSSAHGSQR TSGPESSMKVTSSIPVFDLQDGGRKICPRCNAQFRVTEALRGHMCYCCPE MVEYQKKGKSLDSEPSVPSAAKPPSPEKTAPVASTPSSTPIPALSPPTKV PEPNENVGDAVQTKLIMLVDDFYYGRDGGKVAQLTNFPKVATSFRCPHCT KRLKNNIRFMNHMKHHVELDQQNGEVDGHTICQHCYRQFSTPFQLQCHLE NVHSPYESTTKC
Database link: Q7Z3K3 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.5 -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171934 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000. Predicted molecular weight: 155 kDa.
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ICC/IF |
1/500.
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Notes |
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WB
1/1000. Predicted molecular weight: 155 kDa. |
ICC/IF
1/500. |
Target
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Function
Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms. -
Sequence similarities
Contains 9 C2H2-type zinc fingers.
Contains 1 DDE domain.
Contains 1 HTH CENPB-type DNA-binding domain. -
Cellular localization
Nucleus. Chromosome. Cytoplasm. According to some authors, it is not localized to mitotic chromatin (PubMed:19244240). Recruited to trimethylated 'Lys-9' of histone H3. - Information by UniProt
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Database links
- Entrez Gene: 23126 Human
- Omim: 614787 Human
- SwissProt: Q7Z3K3 Human
- Unigene: 489873 Human
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Alternative names
- KIAA0461 antibody
- MGC71543 antibody
- Pogo transposable element with ZNF domain antibody
see all
Images
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All lanes : Anti-POGZ antibody (ab171934) at 1/1000 dilution
Lane 1 : Crude extract of HeLa cells transfected with control siRNA
Lane 2 : Crude extract of HeLa cells transfected with POGZ siRNA
Predicted band size: 155 kDa -
Immunofluorescenct analysis of HeLa cells (chromosome spread preparations) labeling POGZ with ab171934 at 1/500 dilution and stained with Hoechst 33342.
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Immunofluorescent analysis of Paraformaldehyde-fixed HeLa cells labeling POGZ with ab171934 at 1/500 dilution and stained with Hoechst 33342.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab171934 has been referenced in 2 publications.
- Sun X et al. Autism-associated protein POGZ controls ESCs and ESC neural induction by association with esBAF. Mol Autism 13:24 (2022). PubMed: 35650610
- Lee HS et al. The chromatin remodeler RSF1 controls centromeric histone modifications to coordinate chromosome segregation. Nat Commun 9:3848 (2018). PubMed: 30242288