Overview

  • Product name

    Anti-POGZ antibody [EPR10612]
    See all POGZ primary antibodies
  • Description

    Rabbit monoclonal [EPR10612] to POGZ
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human POGZ aa 1-100.

  • Positive control

    • HepG2 and SH-SY5Y cell lysates; Human cervical carcinoma and Human prostate tissues; HepG2 and SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10612
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 155 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms.
    • Sequence similarities

      Contains 9 C2H2-type zinc fingers.
      Contains 1 DDE domain.
      Contains 1 HTH CENPB-type DNA-binding domain.
    • Cellular localization

      Nucleus. Chromosome. Cytoplasm. According to some authors, it is not localized to mitotic chromatin (PubMed:19244240). Recruited to trimethylated 'Lys-9' of histone H3.
    • Information by UniProt
    • Database links

    • Alternative names

      • KIAA0461 antibody
      • MGC71543 antibody
      • Pogo transposable element with ZNF domain antibody
      • POGZ antibody
      • POGZ_HUMAN antibody
      • putative protein product of Nbla00003 antibody
      • SUHW5 antibody
      • suppressor of hairy wing homolog antibody
      • Suppressor of hairy wing homolog 5 antibody
      • Zinc finger protein 280E antibody
      • Zinc finger protein 635 antibody
      • ZNF280E antibody
      • ZNF635 antibody
      • ZNF635M antibody
      see all

    Images

    • All lanes : Anti-POGZ antibody [EPR10612] (ab167408) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 155 kDa

    • ab167408 showing +ve staining in Human normal colon.
    • ab167408 showing +ve staining in Human breast carcinoma.
    • ab167408 showing +ve staining in Human ovarian carcinoma.
    • Flow cytometric analysis of permeabilized SH-SY5Y cells, labeling POGZ with ab167408 at 1/10 dilution (red) or a Rabbit IgG negative (green).
    • Immunofluorescent staining of HepG2 cells labeling POGZ with ab167408 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling POGZ with ab167408 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling POGZ with ab167408 at 1/50 dilution.

    References

    ab167408 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293 cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    293 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Jun 24 2019

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (Age P0 brain (Subventricular zone))
    Permeabilization
    Yes - 0.05% Triton
    Specification
    Age P0 brain (Subventricular zone)
    Blocking step
    TNB Blocking Buffer from Perkin Elmer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 24 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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