Recombinant Anti-POGZ antibody [EPR10612] (ab167408)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10612] to POGZ
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-POGZ antibody [EPR10612]
See all POGZ primary antibodies -
Description
Rabbit monoclonal [EPR10612] to POGZ -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human POGZ aa 1-100.
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Positive control
- HepG2 and SH-SY5Y cell lysates; Human cervical carcinoma and Human prostate tissues; HepG2 and SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10612 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 155 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/100 - 1/250.
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 155 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Plays a role in mitotic cell cycle progression and is involved in kinetochore assembly and mitotic sister chromatid cohesion. Probably through its association with CBX5 plays a role in mitotic chromosome segregation by regulating aurora kinase B/AURKB activation and AURKB and CBX5 dissociation from chromosome arms. -
Sequence similarities
Contains 9 C2H2-type zinc fingers.
Contains 1 DDE domain.
Contains 1 HTH CENPB-type DNA-binding domain. -
Cellular localization
Nucleus. Chromosome. Cytoplasm. According to some authors, it is not localized to mitotic chromatin (PubMed:19244240). Recruited to trimethylated 'Lys-9' of histone H3. - Information by UniProt
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Database links
- Entrez Gene: 23126 Human
- Entrez Gene: 229584 Mouse
- Entrez Gene: 310658 Rat
- Omim: 614787 Human
- SwissProt: Q7Z3K3 Human
- SwissProt: Q8BZH4 Mouse
- Unigene: 489873 Human
- Unigene: 274787 Mouse
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Alternative names
- KIAA0461 antibody
- MGC71543 antibody
- Pogo transposable element with ZNF domain antibody
see all
Images
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All lanes : Anti-POGZ antibody [EPR10612] (ab167408) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 155 kDa -
ab167408 showing +ve staining in Human normal colon.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab167408 showing +ve staining in Human breast carcinoma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab167408 showing +ve staining in Human ovarian carcinoma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells, labeling POGZ with ab167408 at 1/10 dilution (red) or a Rabbit IgG negative (green).
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Immunofluorescent staining of HepG2 cells labeling POGZ with ab167408 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling POGZ with ab167408 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling POGZ with ab167408 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab167408 has been referenced in 1 publication.
- Hazelbaker DZ et al. A multiplexed gRNA piggyBac transposon system facilitates efficient induction of CRISPRi and CRISPRa in human pluripotent stem cells. Sci Rep 10:635 (2020). PubMed: 31959800