Product nameAnti-Pokemon antibody
See all Pokemon primary antibodies
DescriptionRabbit polyclonal to Pokemon
SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey
Synthetic peptide corresponding to a region between C terminal amino acids 525 - 584 of Human Pokemon. (NP_056982.1)
- Whole cell lysate from HeLa, 293T cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 73 kDa (predicted molecular weight: 62 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'.
Tissue specificityWidely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level).
Sequence similaritiesContains 1 BTB (POZ) domain.
Contains 4 C2H2-type zinc fingers.
- Information by UniProt
- Factor binding IST protein 1 antibody
- Factor that binds to inducer of short transcripts protein 1 antibody
- FBI 1 antibody
All lanes : Anti-Pokemon antibody (ab70208) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 62 kDa
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling Pokemon with ab70208 at 1/200 (1µg/ml). Detection: DAB.
IHC image of Pokemon staining in Human Normal Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70208, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunoprecipitation analysis of HeLa whole cell lysate (1 mg for IP; 1/4 of IP loaded). ab70208 was used at 3 µg/mg lysate for IP and 0.04 µg/ml for WB. Lane 1 - ab70208 IP, Lane 2 - control IgG.
This product has been referenced in:
- Guo C et al. The expression and clinical significance of ZBTB7 in transitional cell carcinoma of the bladder. Oncol Lett 14:4857-4862 (2017). Read more (PubMed: 29085492) »
- Liu F et al. Differences in Zbtb7a expression cause heterogeneous changes in human nasopharyngeal carcinoma CNE3 sublines. Oncol Lett 14:2669-2676 (2017). Read more (PubMed: 28927029) »