Key features and details
- Rabbit polyclonal to POLD1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-POLD1 antibody
See all POLD1 primary antibodies
DescriptionRabbit polyclonal to POLD1
This antibody detects endogenous level of total POLD1, catalytic subunit protein.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment within Human POLD1 aa 1-1107 (C terminal). The exact sequence is proprietary.
Database link: P28340
- HeLa, SW480 and mouse spleen cell extracts: U2OS cells.
This product was previously labelled as DNA Polymerase delta
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 124 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionPossesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.
Sequence similaritiesBelongs to the DNA polymerase type-B family.
- Information by UniProt
- Polymerase (DNA directed) delta 1 catalytic subunit antibody
- CDC2 antibody
- CDC2 homolog antibody
All lanes : Anti-POLD1 antibody (ab196561) at 1/500 dilution
Lane 1 : HeLa cell extract
Lane 2 : SW480 cell extract
Lane 3 : Mouse spleen cell extract
Predicted band size: 124 kDa
Immunofluorescent analysis of U2OS cells labeling POLD1, catalytic subunit with ab196561 at 1/50 dilution. Blue: DAPI for nuclear staining.
ab196561 has been referenced in 1 publication.
- Simon AK et al. The iron-sulfur helicase DDX11 promotes the generation of single-stranded DNA for CHK1 activation. Life Sci Alliance 3:N/A (2020). PubMed: 32071282