Overview

  • Product name

  • Description

    Rabbit polyclonal to POLD1
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous level of total POLD1, catalytic subunit protein.

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment within Human POLD1 aa 1-1107 (C terminal). The exact sequence is proprietary.
    Database link: P28340

  • Positive control

    • HeLa, SW480 and mouse spleen cell extracts: U2OS cells.
  • General notes

     This product was previously labelled as DNA Polymerase delta

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride

    PBS without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab196561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 124 kDa.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.
  • Sequence similarities

    Belongs to the DNA polymerase type-B family.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Polymerase (DNA directed) delta 1 catalytic subunit antibody
    • CDC2 antibody
    • CDC2 homolog antibody
    • CRCS10 antibody
    • DNA directed DNA polymerase delta 1 antibody
    • DNA directed polymerase delta 1 antibody
    • DNA pol delta 1 antibody
    • DNA polymerase delta catalytic subunit antibody
    • DNA polymerase subunit delta p125 antibody
    • DPOD1_HUMAN antibody
    • MDPL antibody
    • POLD antibody
    • POLD 1 antibody
    • POLD1 antibody
    • Polymerase (DNA directed) delta 1 catalytic subunit 125kDa antibody
    • Polymerase (DNA) delta 1 catalytic subunit antibody
    • Polymerase DNA directed delta 1 catalytic subunit 125kD antibody
    • polymerase, DNA, delta antibody
    see all

Images

  • All lanes : Anti-POLD1 antibody (ab196561) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : SW480 cell extract
    Lane 3 : Mouse spleen cell extract

    Predicted band size: 124 kDa

  • Immunofluorescent analysis of U2OS cells labeling POLD1, catalytic subunit with ab196561 at 1/50 dilution. Blue: DAPI for nuclear staining.

References

ab196561 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab196561.
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