Product nameAnti-POLD1 antibody
See all POLD1 primary antibodies
DescriptionRabbit polyclonal to POLD1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Hamster, Cow
Synthetic peptide within Human POLD1 aa 930-980. The exact sequence is proprietary. NP_002682.2
Database link: P28340
- WB: HeLa, HEK-293T, Jurkat, TCMK1 and NIH/3T3 whole cell lysates. IP: Jurkat whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/25000. Predicted molecular weight: 124 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPossesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.
Sequence similaritiesBelongs to the DNA polymerase type-B family.
- Information by UniProt
- Polymerase (DNA directed) delta 1 catalytic subunit antibody
- CDC2 antibody
- CDC2 homolog antibody
All lanes : Anti-POLD1 antibody (ab264345) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 30 seconds
Lysates prepared using NETN lysis buffer.
POLD1 was immunoprecipitated from Jurkat whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264345 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264345 at 1 μg/ml.
Lane 1: ab264345 IP in Jurkay whole cell lysate.
Lane 2: ab264345 IP in Jurkay whole cell lysate.
Lane 3: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
Different batches used for IP in lanes 1 and 2.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264345 has not yet been referenced specifically in any publications.