Overview

  • Product name

    Anti-POLD2 antibody - C-terminal
    See all POLD2 primary antibodies
  • Description

    Rabbit polyclonal to POLD2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human POLD2 aa 419-469 (C terminal). The exact sequence is proprietary.
    Sequence:

    EDQTVLLVTVPDFSATQTACLVNLRSLACQPISFSGFGAEDDDLGGLGLG P


    Database link: P49005

  • Positive control

    • HeLa, 293T and Jurkat whole cell lysates.
  • General notes

     This product was previously labelled as DNA polymerase delta p50

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187710 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 51 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-POLD2 antibody - C-terminal (ab187710) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 51 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation of POLD2 from 293T whole cell lysate (1 mg per lane), using ab187710 at 6 µg (lane 1) or an IgG control (lane 2). For western blot of immunoprecipitated protein, ab187710 was used at 1 µg/ml.

    Detection: ECL with an exposure time of 30 seconds.

References

ab187710 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Chicken Cell lysate - whole cell (DT40)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
DT40
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 12 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up