Recombinant Anti-POLD3 antibody [EP9480] (ab182564)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP9480] to POLD3
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-POLD3 antibody [EP9480]
See all POLD3 primary antibodies -
Description
Rabbit monoclonal [EP9480] to POLD3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, 293T and Jurkat lysates. ICC: 293T cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP9480 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 51 kDa).
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ICC/IF |
1/100.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 51 kDa). |
ICC/IF
1/100. |
Target
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Function
Required for optimal DNA polymerase delta activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10714 Human
- Omim: 611415 Human
- SwissProt: Q15054 Human
- Unigene: 82502 Human
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Alternative names
- DNA polymerase delta subunit 3 antibody
- DNA polymerase delta subunit p66 antibody
- DNA polymerase delta, subunit 3 antibody
see all
Images
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Anti-POLD3 antibody [EP9480] (ab182564) at 1/5000 dilution + Jurkat lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted? -
All lanes : Anti-POLD3 antibody [EP9480] (ab182564) at 1/5000 dilution
Lane 1 : Hela lysate
Lane 2 : 293T lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted? -
Immunofluorescent analysis of 4% paraformaldehyde-fixed 293T cells labeling POLD3 with ab182564 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®555) secondary antibody at 1/200 dilution (left panel). Right panel counter stained with Dapi (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (6)
ab182564 has been referenced in 6 publications.
- Lippert TP et al. Oncogenic herpesvirus KSHV triggers hallmarks of alternative lengthening of telomeres. Nat Commun 12:512 (2021). PubMed: 33479235
- Li S et al. PIF1 helicase promotes break-induced replication in mammalian cells. EMBO J 40:e104509 (2021). PubMed: 33470420
- Tan J et al. An R-loop-initiated CSB-RAD52-POLD3 pathway suppresses ROS-induced telomeric DNA breaks. Nucleic Acids Res 48:1285-1300 (2020). PubMed: 31777915
- Pinzaru AM et al. Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore. Genes Dev 34:1619-1636 (2020). PubMed: 33122293
- Hu Q et al. Break-induced replication plays a prominent role in long-range repeat-mediated deletion. EMBO J 38:e101751 (2019). PubMed: 31571254
- Ren L et al. Potential biomarkers of DNA replication stress in cancer. Oncotarget 8:36996-37008 (2017). PubMed: 28445142