Recombinant Anti-POLDIP2 antibody [EPR13832] (ab181841)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13832] to POLDIP2
- Suitable for: WB, Flow Cyt, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-POLDIP2 antibody [EPR13832]
See all POLDIP2 primary antibodies -
Description
Rabbit monoclonal [EPR13832] to POLDIP2 -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanIP HumanWB Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and HepG2 cell lysates. Flow Cytometry: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13832 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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IP |
Human
|
WB |
Human
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All applications |
Mouse
Rat
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Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 42 kDa).
|
Flow Cyt |
1/350.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
IP |
1/40.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 42 kDa). |
Flow Cyt
1/350. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
IP
1/40. |
Target
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Sequence similarities
Contains 1 apaG domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 26073 Human
- Entrez Gene: 67811 Mouse
- Entrez Gene: 287544 Rat
- Omim: 611519 Human
- SwissProt: Q9Y2S7 Human
- SwissProt: Q91VA6 Mouse
- Unigene: 241543 Human
- Unigene: 197493 Mouse
see all -
Alternative names
- 38 kDa DNA polymerase delta interaction protein antibody
- delta interacting protein 2 antibody
- DNA polymerase delta interaction protein p38 antibody
see all
Images
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: POLDIP2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab181841 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab181841 was shown to specifically recognize POLDIP2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when POLDIP2 knockout samples were examined. Wild-type and POLDIP2 knockout samples were subjected to SDS-PAGE. ab181841 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling POLDIP2 with ab181841 at 1/350 diltuion (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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All lanes : Anti-POLDIP2 antibody [EPR13832] (ab181841) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted? -
Western blot analysis of HeLa cell lysate immunoprecipitated with ab181841 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab181841 has been referenced in 4 publications.
- Forrester SJ et al. Poldip2 deficiency protects against lung edema and vascular inflammation in a model of acute respiratory distress syndrome. Clin Sci (Lond) 133:321-334 (2019). PubMed: 30622219
- Kikuchi DS et al. Poldip2 mediates blood-brain barrier disruption in a model of sepsis-associated encephalopathy. J Neuroinflammation 16:241 (2019). PubMed: 31779628
- Hernandes MS et al. Polymerase delta-interacting protein 2 deficiency protects against blood-brain barrier permeability in the ischemic brain. J Neuroinflammation 15:45 (2018). PubMed: 29452577
- Li MS et al. NADPH oxidase-2 mediates zinc deficiency-induced oxidative stress and kidney damage. Am J Physiol Cell Physiol 312:C47-C55 (2017). PubMed: 27806940