Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-POLDIP2 antibody [EPR13832] (ab181841)

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Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841)
  • Flow Cytometry - Anti-POLDIP2 antibody [EPR13832] (ab181841)
  • Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841)
  • Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] (ab181841)
  • Anti-POLDIP2 antibody [EPR13832] (ab181841)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13832] to POLDIP2
  • Suitable for: WB, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-POLDIP2 antibody [EPR13832]
    See all POLDIP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13832] to POLDIP2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and HepG2 cell lysates. Flow Cytometry: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13832

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IP
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 42 kDa).
Flow Cyt
1/350.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP
1/40.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 42 kDa).
Flow Cyt
1/350.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IP
1/40.

Target

Images

  • Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841)
    Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: POLDIP2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab181841 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab181841 was shown to specifically recognize POLDIP2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when POLDIP2 knockout samples were examined. Wild-type and POLDIP2 knockout samples were subjected to SDS-PAGE. ab181841 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

     

  • Flow Cytometry - Anti-POLDIP2 antibody [EPR13832] (ab181841)
    Flow Cytometry - Anti-POLDIP2 antibody [EPR13832] (ab181841)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling POLDIP2 with ab181841 at 1/350 diltuion (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841)
    Western blot - Anti-POLDIP2 antibody [EPR13832] (ab181841)
    All lanes : Anti-POLDIP2 antibody [EPR13832] (ab181841) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] (ab181841)
    Immunoprecipitation - Anti-POLDIP2 antibody [EPR13832] (ab181841)

    Western blot analysis of HeLa cell lysate immunoprecipitated with ab181841 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.

  • Anti-POLDIP2 antibody [EPR13832] (ab181841)
    Anti-POLDIP2 antibody [EPR13832] (ab181841)

References (4)

Publishing research using ab181841? Please let us know so that we can cite the reference in this datasheet.

ab181841 has been referenced in 4 publications.

  • Forrester SJ  et al. Poldip2 deficiency protects against lung edema and vascular inflammation in a model of acute respiratory distress syndrome. Clin Sci (Lond) 133:321-334 (2019). PubMed: 30622219
  • Kikuchi DS  et al. Poldip2 mediates blood-brain barrier disruption in a model of sepsis-associated encephalopathy. J Neuroinflammation 16:241 (2019). PubMed: 31779628
  • Hernandes MS  et al. Polymerase delta-interacting protein 2 deficiency protects against blood-brain barrier permeability in the ischemic brain. J Neuroinflammation 15:45 (2018). PubMed: 29452577
  • Li MS  et al. NADPH oxidase-2 mediates zinc deficiency-induced oxidative stress and kidney damage. Am J Physiol Cell Physiol 312:C47-C55 (2017). PubMed: 27806940

Customer reviews and Q&As

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Western blot abreview for Anti-POLDIP2 antibody [EPR13832]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293 cells)
Gel Running Conditions
Reduced Denaturing (12 % gel)
Loading amount
1 µg
Specification
293 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Ko Suzuki

Verified customer

Submitted Jul 26 2019

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