Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free (ab232609)

Overview

  • Product name

    Anti-POLDIP2 antibody [EPR13832] - BSA and Azide free
    See all POLDIP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR13832] to POLDIP2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human POLDIP2 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9Y2S7

  • Positive control

    • WB: Wild-type HAP1 whole cell lysate. HeLa and HepG2 whole cell lysate.
  • General notes

    Ab232609 is the carrier-free version of ab181841. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab232609 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13832
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

Images

  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: POLDIP2 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HepG2 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab181841 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab181841 was shown to specifically recognize POLDIP2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when POLDIP2 knockout samples were examined. Wild-type and POLDIP2 knockout samples were subjected to SDS-PAGE. ab181841 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1,000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181841).

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling POLDIP2 with ab181841 at 1/350 diltuion (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181841).

  • Western blot analysis of HeLa cell lysate immunoprecipitated with ab181841 at 1/40 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181841).

References

ab232609 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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