Anti-POLE antibody (ab110876)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-POLE antibody
See all POLE primary antibodies -
DescriptionRabbit polyclonal to POLE
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide derived from an internal region of Human POLE.
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Positive control
- Human breast carcinoma tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab110876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionParticipates in DNA repair and in chromosomal DNA replication.
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Sequence similaritiesBelongs to the DNA polymerase type-B family.
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DomainThe DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C. The C-terminus may also regulate the catalytic activities of the enzyme.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 5426 Human
- Entrez Gene: 18973 Mouse
- Omim: 174762 Human
- SwissProt: Q07864 Human
- SwissProt: Q9WVF7 Mouse
- Unigene: 524871 Human
- Unigene: 35061 Mouse
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Alternative names
- CRCS12 antibody
- DKFZp434F222 antibody
- DNA polymerase epsilon catalytic subunit A antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody (ab110876)ab110876, at a 1/50 dilution, staining POLE in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry, in the absence (left panel) or presence (right panel) of the immunizing peptide.
Protocols
References
This product has been referenced in:
- Elsayed FA et al. Germline variants in POLE are associated with early onset mismatch repair deficient colorectal cancer. Eur J Hum Genet N/A:N/A (2014). Read more (PubMed: 25370038) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"