Anti-POLE antibody (ab110876)
Key features and details
- Rabbit polyclonal to POLE
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-POLE antibody
See all POLE primary antibodies -
Description
Rabbit polyclonal to POLE -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide derived from an internal region of Human POLE.
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Positive control
- Human breast carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110876 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Participates in DNA repair and in chromosomal DNA replication. -
Sequence similarities
Belongs to the DNA polymerase type-B family. -
Domain
The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C. The C-terminus may also regulate the catalytic activities of the enzyme. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5426 Human
- Entrez Gene: 18973 Mouse
- Omim: 174762 Human
- SwissProt: Q07864 Human
- SwissProt: Q9WVF7 Mouse
- Unigene: 524871 Human
- Unigene: 35061 Mouse
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Alternative names
- CRCS12 antibody
- DKFZp434F222 antibody
- DNA polymerase epsilon catalytic subunit A antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody (ab110876)ab110876, at a 1/50 dilution, staining POLE in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry, in the absence (left panel) or presence (right panel) of the immunizing peptide.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab110876 has been referenced in 1 publication.
- Elsayed FA et al. Germline variants in POLE are associated with early onset mismatch repair deficient colorectal cancer. Eur J Hum Genet N/A:N/A (2014). PubMed: 25370038