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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Nucl. Excision Repair
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Anti-POLE antibody (ab110876)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody (ab110876)

    Key features and details

    • Rabbit polyclonal to POLE
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human POLE protein (ab159152)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-POLE antibody
      See all POLE primary antibodies
    • Description

      Rabbit polyclonal to POLE
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse
    • Immunogen

      Synthetic peptide derived from an internal region of Human POLE.

    • Positive control

      • Human breast carcinoma tissue
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • Nucl. Excision Repair
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Synthesis
      • DNA Polymerases

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human POLE protein (ab159152)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab110876 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Participates in DNA repair and in chromosomal DNA replication.
    • Sequence similarities

      Belongs to the DNA polymerase type-B family.
    • Domain

      The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C. The C-terminus may also regulate the catalytic activities of the enzyme.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q07864 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5426 Human
      • Entrez Gene: 18973 Mouse
      • Omim: 174762 Human
      • SwissProt: Q07864 Human
      • SwissProt: Q9WVF7 Mouse
      • Unigene: 524871 Human
      • Unigene: 35061 Mouse
      • Alternative names

        • CRCS12 antibody
        • DKFZp434F222 antibody
        • DNA polymerase epsilon catalytic subunit A antibody
        • DNA polymerase epsilon catalytic subunit antibody
        • DNA polymerase II subunit A antibody
        • DPOE1_HUMAN antibody
        • EC 2.7.7.7 antibody
        • FILS antibody
        • FLJ21434 antibody
        • POLE antibody
        • POLE1 antibody
        • Polymerase (DNA directed) epsilon antibody
        • polymerase (DNA directed), epsilon, catalytic subunit antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody (ab110876)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody (ab110876)
        ab110876, at a 1/50 dilution, staining POLE in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry, in the absence (left panel) or presence (right panel) of the immunizing peptide.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab110876? Please let us know so that we can cite the reference in this datasheet.

      ab110876 has been referenced in 1 publication.

      • Elsayed FA  et al. Germline variants in POLE are associated with early onset mismatch repair deficient colorectal cancer. Eur J Hum Genet N/A:N/A (2014). PubMed: 25370038

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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