Anti-POLE antibody (ab110876)

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Overview

  • Product name

    Anti-POLE antibody
    See all POLE primary antibodies

  • Description

    Rabbit polyclonal to POLE

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide derived from an internal region of Human POLE.

  • Positive control

    • Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab110876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Participates in DNA repair and in chromosomal DNA replication.

  • Sequence similarities

    Belongs to the DNA polymerase type-B family.

  • Domain

    The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C. The C-terminus may also regulate the catalytic activities of the enzyme.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • CRCS12 antibody
      • DKFZp434F222 antibody
      • DNA polymerase epsilon catalytic subunit A antibody
      • DNA polymerase epsilon catalytic subunit antibody
      • DNA polymerase II subunit A antibody
      • DPOE1_HUMAN antibody
      • EC 2.7.7.7 antibody
      • FILS antibody
      • FLJ21434 antibody
      • POLE antibody
      • POLE1 antibody
      • Polymerase (DNA directed) epsilon antibody
      • polymerase (DNA directed), epsilon, catalytic subunit antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody (ab110876)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-POLE antibody (ab110876)
      ab110876, at a 1/50 dilution, staining POLE in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry, in the absence (left panel) or presence (right panel) of the immunizing peptide.

    References

    This product has been referenced in:

    • Elsayed FA  et al. Germline variants in POLE are associated with early onset mismatch repair deficient colorectal cancer. Eur J Hum Genet N/A:N/A (2014). Read more (PubMed: 25370038) »
    See 1 Publication for this product

    Publishing research using ab110876? Please let us know so that we can cite the reference in this datasheet.

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