Key features and details
- Mouse polyclonal to POLE
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab67420 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
FunctionParticipates in DNA repair and in chromosomal DNA replication.
Sequence similaritiesBelongs to the DNA polymerase type-B family.
DomainThe DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C. The C-terminus may also regulate the catalytic activities of the enzyme.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CRCS12 antibody
- DKFZp434F222 antibody
- DNA polymerase epsilon catalytic subunit A antibody
All lanes : Anti-POLE antibody (ab67420) at 1/500 dilution
Lane 1 : POLE transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg/ml per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
ab67420 has not yet been referenced specifically in any publications.