Overview

  • Product name

    Anti-POLE antibody - N-terminal
    See all POLE primary antibodies
  • Description

    Rabbit polyclonal to POLE - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment within Human POLE (N terminal). The exact sequence is proprietary.
    Database link: Q07864

  • Positive control

    • ICC/IF: HeLa cells. IHC-P: Human ovary tissue. WB: K562 whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab226848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 262 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Participates in DNA repair and in chromosomal DNA replication.
  • Sequence similarities

    Belongs to the DNA polymerase type-B family.
  • Domain

    The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C. The C-terminus may also regulate the catalytic activities of the enzyme.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CRCS12 antibody
    • DKFZp434F222 antibody
    • DNA polymerase epsilon catalytic subunit A antibody
    • DNA polymerase epsilon catalytic subunit antibody
    • DNA polymerase II subunit A antibody
    • DPOE1_HUMAN antibody
    • EC 2.7.7.7 antibody
    • FILS antibody
    • FLJ21434 antibody
    • POLE antibody
    • POLE1 antibody
    • Polymerase (DNA directed) epsilon antibody
    • polymerase (DNA directed), epsilon, catalytic subunit antibody
    see all

Images

  • Anti-POLE antibody - N-terminal (ab226848) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract at 30 µg

    Predicted band size: 262 kDa



    5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling POLE (green) using ab226848 at 1/2500 dilution in ICC/IF. Red: phalloidin, a cytoskeleton marker at 1/100 dilution.

  • Paraffin-embedded human ovary tissue stained for POLE using ab226848 at 1/500 dilution in immunohistochemical analysis.

References

ab226848 has not yet been referenced specifically in any publications.

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