Key features and details
- Rabbit polyclonal to POLE - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-POLE antibody - N-terminal
See all POLE primary antibodies
DescriptionRabbit polyclonal to POLE - N-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment within Human POLE (N terminal). The exact sequence is proprietary.
Database link: Q07864
- ICC/IF: HeLa cells. IHC-P: Human ovary tissue. WB: K562 whole cell extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 262 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionParticipates in DNA repair and in chromosomal DNA replication.
Sequence similaritiesBelongs to the DNA polymerase type-B family.
DomainThe DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C. The C-terminus may also regulate the catalytic activities of the enzyme.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- CRCS12 antibody
- DKFZp434F222 antibody
- DNA polymerase epsilon catalytic subunit A antibody
Anti-POLE antibody - N-terminal (ab226848) at 1/1000 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract at 30 µg
Predicted band size: 262 kDa
5% SDS-PAGE gel.
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling POLE (green) using ab226848 at 1/2500 dilution in ICC/IF. Red: phalloidin, a cytoskeleton marker at 1/100 dilution.
Paraffin-embedded human ovary tissue stained for POLE using ab226848 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226848 has not yet been referenced specifically in any publications.