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Epigenetics and Nuclear Signaling DNA / RNA DNA Synthesis DNA Polymerases
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RecombinantRabMAb

Recombinant Anti-POLG antibody [EPR7295] (ab128862)

  • Datasheet
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Western blot - Anti-POLG antibody [EPR7295] (ab128862)
  • OI-RD Scanning - Anti-POLG antibody [EPR7295] (ab128862)
  • Anti-POLG antibody [EPR7295] (ab128862)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7295] to POLG
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-POLG antibody [EPR7295]
    See all POLG primary antibodies
  • Description

    Rabbit monoclonal [EPR7295] to POLG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human POLG aa 50-150. The exact sequence is proprietary.

  • Positive control

    • 293T, HeLa, MCF7 and HepG2 cell lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as DNA Polymerase gamma

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.61 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7295
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Synthesis
    • DNA Polymerases
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial Biogenesis
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Molecular processes
    • Mitochondrial transcription

Associated products

  • Alternative Versions

    • Anti-POLG antibody [EPR7295] - BSA and Azide free (ab248192)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human POLG protein (ab196066)

Applications

Our Abpromise guarantee covers the use of ab128862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 140 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Involved in the replication of mitochondrial DNA.
    • Involvement in disease

      Defects in POLG are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 1 (PEOA1) [MIM:157640]. Progressive external ophthalmoplegia is characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism.
      Defects in POLG are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal recessive (PEOB) [MIM:258450]. PEOB is a severe form of progressive external ophthalmoplegia. It is clinically more heterogeneous than the autosomal dominant forms. Can be more severe.
      Defects in POLG are a cause of sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO) [MIM:607459]. SANDO is a clinically heterogeneous systemic disorder with variable features resulting from mitochondrial dysfunction. It shares phenotypic characteristics with autosomal recessive progressive external ophthalmoplegia and mitochondrial neurogastrointestinal encephalopathy syndrome. The clinical triad of symptoms consists of sensory ataxic, neuropathy, dysarthria, and ophthalmoparesis.
      Defects in POLG are a cause of Alpers-Huttenlocher syndrome (AHS) [MIM:203700]; also called Alpers diffuse degeneration of cerebral gray matter with hepatic cirrhosis. AHS is an autosomal recessive hepatocerebral syndrome. The typical course of AHS includes severe developmental delay, intractable seizures, liver failure, and death in childhood. Refractory seizures, cortical blindness, progressive liver dysfunction, and acute liver failure after exposure to valproic acid are considered diagnostic features. The neuropathological hallmarks of AHS are neuronal loss, spongiform degeneration, and astrocytosis of the visual cortex. Liver biopsy results show steatosis, often progressing to cirrhosis.
      Defects in POLG are a cause of mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE) [MIM:603041]; also known as myoneurogastrointestinal encephalomyopathy. MNGIE is an autosomal recessive disease associated with multiple deletions of skeletal muscle mitochondrial DNA (MtDNA). It is clinically characterized by onset between the second and fifth decades of life, ptosis, progressive external ophthalmoplegia, gastrointestinal dysmotility (often pseudoobstruction), diffuse leukoencephalopathy, thin body habitus, peripheral neuropathy, and myopathy.
      Defects in POLG are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
    • Sequence similarities

      Belongs to the DNA polymerase type-A family.
    • Cellular localization

      Mitochondrion.
    • Target information above from: UniProt accession P54098 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5428 Human
      • Omim: 174763 Human
      • SwissProt: P54098 Human
      • Unigene: 706868 Human
      • Alternative names

        • DNA directed DNA polymerase gamma antibody
        • DNA polymerase subunit gamma 1 antibody
        • DNA polymerase subunit gamma-1 antibody
        • DPOG1_HUMAN antibody
        • MDP 1 antibody
        • MDP1 antibody
        • Mitochondrial DNA polymerase catalytic subunit antibody
        • Mitochondrial DNA polymerase gamma antibody
        • PEO antibody
        • POLG 1 antibody
        • POLG A antibody
        • PolG alpha antibody
        • POLG antibody
        • PolG-alpha antibody
        • POLG1 antibody
        • POLGA antibody
        • Polymerase (DNA directed) gamma antibody
        • SANDO antibody
        • SCAE antibody
        see all

      Images

      • Western blot - Anti-POLG antibody [EPR7295] (ab128862)
        Western blot - Anti-POLG antibody [EPR7295] (ab128862)
        All lanes : Anti-POLG antibody [EPR7295] (ab128862) at 1/1000 dilution

        Lane 1 : 293T cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : MCF7 cell lysate
        Lane 4 : HepG2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 140 kDa

      • OI-RD Scanning - Anti-POLG antibody [EPR7295] (ab128862)
        OI-RD Scanning - Anti-POLG antibody [EPR7295] (ab128862)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-POLG antibody [EPR7295] (ab128862)
        Anti-POLG antibody [EPR7295] (ab128862)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab128862? Please let us know so that we can cite the reference in this datasheet.

      ab128862 has been referenced in 2 publications.

      • Gong Y  et al. Tetratricopeptide repeat domain 3 overexpression tends to form aggregates and inhibit ubiquitination and degradation of DNA polymerase ?. Oncotarget 8:106475-106485 (2017). PubMed: 29290964
      • Kim DM  et al. Identification of a Mitochondrial DNA Polymerase Affecting Cardiotoxicity of Sunitinib Using a Genome-Wide Screening on S. pombe Deletion Library. Toxicol Sci 149:4-14 (2016). PubMed: 26385865

      Customer reviews and Q&As

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      Please use the links above to contact us or submit feedback about this product.

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