Overview

  • Product name

    Anti-Poliovirus Receptor/PVR antibody
    See all Poliovirus Receptor/PVR primary antibodies
  • Description

    Rabbit polyclonal to Poliovirus Receptor/PVR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length protein corresponding to Human Poliovirus Receptor/PVR aa 1-417.
    Database link: NP_006496.2

  • Positive control

    • Mouse stomach tissue lysate.
  • General notes

     This product was previously labelled as Poliovirus Receptor

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 45 kDa.
ICC/IF 1/200.

Target

  • Function

    Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.
  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD155 antibody
    • CD155 antigen antibody
    • FLJ25946 antibody
    • HVED antibody
    • mE4 antibody
    • NECL 5 antibody
    • Necl-5 antibody
    • Necl5 antibody
    • Nectin like 5 antibody
    • Nectin like protein 5 antibody
    • Nectin-like protein 5 antibody
    • Ortholog of mouse Tage4 antibody
    • Poliovirus receptor antibody
    • PVR antibody
    • PVR_HUMAN antibody
    • PVS antibody
    • Taa1 antibody
    • Tage 4 antibody
    • TAGE4 antibody
    see all

Images

  • Anti-Poliovirus Receptor/PVR antibody (ab103630) at 1/500 dilution + Mouse stomach tissue lysate at 50 µg

    Predicted band size: 45 kDa

  • All lanes : Anti-Poliovirus Receptor/PVR antibody (ab103630) at 1/500 dilution

    Lane 1 : Poliovirus Receptor/PVR transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 45 kDa

  • Immunofluorescence analysis of murine stomach, staining Poliovirus Receptor/PVR with ab103630.

    Cells were fixed with acetone and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in diluent) for 24 hours at 4°C. A Cy3®-conjugated sheep anti-rabbit polyclonal IgG (1/3000) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (A431 cells)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
10 µg
Specification
A431 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Aug 16 2017

Application
Western blot
Loading amount
35 µg
Gel Running Conditions
Reduced Denaturing
Sample
Pig Cell lysate - whole cell (Swine kidney)
Specification
Swine kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Korneel Grauwet

Verified customer

Submitted Oct 07 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Stomach)
Specification
Stomach
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 18 2012

Answer

DISCOUNT CODE: 42351

Expiration date: 27/12/2012


I am very pleased to hear you would like to accept our offer and testab103630 in IHC-Fr on Ms stomack. This code will give you: 1 free PRIMARY ANTIBODY/PROTEIN OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview forMs stomack and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your answer.

It is very important to design a good control experiments when two untested factors are involved. Could you please confirm ifyou are going to useformalin-fixed paraffin-embedded(IHC-P) or frozen tissue (IHC-Fr) sections (mouse stomach). ICC/IF applies for immunofluorescence on cells (cultured cells or cytospin preparations) only - not on tissue sections.

We need to clarify the application before arranging the discount code.

I look forward to hearing from you soon.

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Answer

Thank you for getting back to me.

This antibody has only been tested in Western blot application on tissue lysate of mouse stomach and transfected human cells (293T). It is very important to control the testing experiments to make sure that the results can be interpreted correctly.

I would suggest using the same type human cells if it is possible. Are these cells HEC1A, ECC1 and Ishikawa transfected as well? Has the expressed levels Poliovirus Receptor been checked previously in them?

You may find some useful infromation about the expression of this target protein at this site:

http://www.proteinatlas.org/ENSG00000073008

As I have explained previously, we can offer testing discount only for either new species or for new application but not for both at the same time since this would involve testing two unknown factors/parameters together.

I hope this will be useful for you.Let me know how you wish to set up your controls for testing two non-tested parameters.

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Answer

Thank you for your enquiry and your interest in our products.

This antibody (ab103630: Anti-Poliovirus Receptor antibody) has not been tested in rat neither in immunohistochemistry.

We can offer testing discount only for either new species or for new application but not for both at the same time since this would involve testing two unknown factors/parameters together.

I would advise you to visit the following site where the Terms and Conditions of our testing initiative can be found at: https://www.abcam.com/collaborationdiscount.

If you need any further assistance in the future, please do not hesitate to contact me.

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