Western blot abreview for Anti-Poliovirus Receptor antibody

Western blot

35 µg

Reduced Denaturing

Pig Cell lysate - whole cell (Swine kidney)

Swine kidney

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

16 hour(s) and 0 minute(s) · Temperature: 4°C

1/250

Non-Abcam antibody was used: goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

1/2000

ECL sirius

15 minute(s) and 0 second(s)

cell lysates of human cell line 293T cells (35 ´g, band was observed)

Thank you for your abreview and for participating in our Abtrial testing program. I am sorry to see that the product did not work. The product has not been tested with pig sample before. And, the immunogen has 51% of sequence homology with the pig protein. It could be unsuitable for use. However, further testing maybe needed to confirm this.