Application
Western blot
Sample
Human Cell lysate - whole cell (A431 cells)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
10 µg
Specification
A431 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% Skim milk
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: goat Anti-Rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 70, 80 kDa
Abcam user community
Verified customer
Submitted Aug 16 2017