Key features and details
- Rabbit polyclonal to Poliovirus Receptor/PVR
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Poliovirus Receptor/PVR antibody
See all Poliovirus Receptor/PVR primary antibodies
DescriptionRabbit polyclonal to Poliovirus Receptor/PVR
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human Poliovirus Receptor/PVR aa 220-345. NP_006496.4
Database link: P15151
- WB: SW620, HT-29, SKOV3, HepG2 and HeLa whole cell lysate; mouse brain and liver tissue lysate. IHC-P: Human prostate and heart tissue.
This product was previously labelled as Poliovirus Receptor
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein A purified
- HeLa whole cell lysate (ab150035)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- HepG2 whole cell lysate (ab166833)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Mouse liver tissue lysate - total protein (ab29301)
- HeLa whole cell lysate (ab29545)
- Mouse brain tissue lysate - total protein (ab30151)
- HT29 whole cell lysate (ab3952)
- Mouse brain tissue lysate - total protein (ab4022)
Our Abpromise guarantee covers the use of ab230338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 45 kDa.|
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionMediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.
Sequence similaritiesBelongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD155 antibody
- CD155 antigen antibody
- FLJ25946 antibody
All lanes : Anti-Poliovirus Receptor/PVR antibody (ab230338) at 1/500 dilution
Lane 1 : SW620 whole cell lysate
Lane 2 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 3 : H460 whole cell lysate
Lane 4 : SKOV3 whole cell lysate
Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 7 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 8 : Mouse brain tissue lysate
Lane 9 : Mouse liver tissue lysate
Predicted band size: 45 kDa
Paraffin-embedded, formalin-fixed human prostate tissue stained for Poliovirus Receptor/PVR using ab230338 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded, formalin-fixed human heart tissue stained for Poliovirus Receptor/PVR using ab230338 at 1/200 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230338 has not yet been referenced specifically in any publications.