Recombinant Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
![Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)](https://www.abcam.com/ps/products/205/ab205304/Images/ab205304-397514-anti-poliovirus-receptorpvr-antibody-epr17302-western-blot-hek-293t-a549-lysates.jpg "Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17302] to Poliovirus Receptor/PVR
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Poliovirus Receptor/PVR antibody [EPR17302]
See all Poliovirus Receptor/PVR primary antibodies -
Description
Rabbit monoclonal [EPR17302] to Poliovirus Receptor/PVR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, HT1080, U-87 MG, A549, HUVEC and K562 whole cell lysates; human fetal heart, fetal kidney and fetal spleen lysates. IP: U-87 MG whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17302 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa).
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| IP |
1/30.
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa). |
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IP
1/30. |
Target
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Function
Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. -
Sequence similarities
Belongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 5817 Human
- Omim: 173850 Human
- SwissProt: P15151 Human
- Unigene: 171844 Human
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Alternative names
- CD155 antibody
- CD155 antigen antibody
- FLJ25946 antibody
see all
Images
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Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PVR knockout HEK-293T cell lysate
Lane 3 : Wild-type A549 cell lysate
Lane 4 : PVR knockout A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Lanes 1- 4: Merged signal (red and green). Green - ab205304 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab205304 was shown to react with Poliovirus Receptor/PVR in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266102 (knockout cell lysate ab257622) was used. Wild-type HEK-293T and PVR knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab205304 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)](https://www.abcam.com/ps/products/205/ab205304/Images/ab205304-252005-anti-poliovirus-receptor-antibody-epr17302-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)Poliovirus Receptor/PVR was immunoprecipitated from 1mg of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with ab205304 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205304 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: U-87 MG whole cell lysate 10µg (Input).
Lane 2: ab205304 IP in U-87 MG whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205304 in U-87 MG whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds. -
![Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)](https://www.abcam.com/ps/products/205/ab205304/Images/ab205304-347008-anti-poliovirus-receptor-antibody-epr17302-westernblot-knock-out-human.jpg)
Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : PVR knockout A549 whole cell lysate
Lane 3 : U87-MG whole cell lysate
Lane 4 : HUVEC whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 45 kDaLanes 1 - 4: Merged signal (red and green). Green - ab205304 observed at 70 kDa (ab205304), 60-80 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab205304 was shown to specifically react with Poliovirus Receptor in wild-type A549 cells as signal was lost in PVR knockout cells. Wild-type and PVR knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab205304 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)](https://www.abcam.com/ps/products/205/ab205304/Images/ab205304-252009-anti-poliovirus-receptor-antibody-epr17302-western-blot.jpg)
Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/4000 dilution
Lane 1 : HT1080 (Human fibrosarcoma cell line) whole cell lysate
Lane 2 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)](https://www.abcam.com/ps/products/205/ab205304/Images/ab205304-252008-anti-poliovirus-receptor-antibody-epr17302-western-blot.jpg)
Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution + K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)](https://www.abcam.com/ps/products/205/ab205304/Images/ab205304-252007-anti-poliovirus-receptor-antibody-epr17302-western-blot.jpg)
Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)](https://www.abcam.com/ps/products/205/ab205304/Images/ab205304-252006-anti-poliovirus-receptor-antibody-epr17302-western-blot.jpg)
Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution
Lane 1 : Untreated K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate deglycosylation (PNGase F) treated
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)](https://www.abcam.com/ps/products/205/ab205304/Images/ab205304-9-benefits-of-recombinant-antibodies.png)
Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab205304 has not yet been referenced specifically in any publications.