Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)

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Overview

  • Product name

    Anti-Poliovirus Receptor/PVR antibody [EPR17302]
    See all Poliovirus Receptor/PVR primary antibodies

  • Description

    Rabbit monoclonal [EPR17302] to Poliovirus Receptor/PVR

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Poliovirus Receptor/PVR aa 200-350. The exact sequence is proprietary.
    Database link: P15151

  • Positive control

    • WB: HT1080, U-87 MG, A549, HUVEC and K562 whole cell lysates; human fetal heart, fetal kidney and fetal spleen lysates. IP: U-87 MG whole cell lysate.

  • General notes

     

    This product was previously labelled as Poliovirus Receptor

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17302

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa).
IP 1/30.

Target

  • Function

    Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.

  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD155 antibody
      • CD155 antigen antibody
      • FLJ25946 antibody
      • HVED antibody
      • mE4 antibody
      • NECL 5 antibody
      • Necl-5 antibody
      • Necl5 antibody
      • Nectin like 5 antibody
      • Nectin like protein 5 antibody
      • Nectin-like protein 5 antibody
      • Ortholog of mouse Tage4 antibody
      • Poliovirus receptor antibody
      • PVR antibody
      • PVR_HUMAN antibody
      • PVS antibody
      • Taa1 antibody
      • Tage 4 antibody
      • TAGE4 antibody
      see all

    Images

    • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution

      Lane 1 : Wild-type A549 whole cell lysate
      Lane 2 : PVR knockout A549 whole cell lysate
      Lane 3 : U87-MG whole cell lysate
      Lane 4 : HUVEC whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 45 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab205304 observed at 70 kDa (ab205304), 60-80 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab205304 was shown to specifically react with Poliovirus Receptor in wild-type A549 cells as signal was lost in PVR knockout cells. Wild-type and PVR knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab205304 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/4000 dilution

      Lane 1 : HT1080 (Human fibrosarcoma cell line) whole cell lysate
      Lane 2 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
      Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Predicted band size: 45 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution + K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Predicted band size: 45 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : Human fetal spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 45 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution

      Lane 1 : Untreated K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
      Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate deglycosylation (PNGase F) treated

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 45 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Immunoprecipitation - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)

      Poliovirus Receptor/PVR was immunoprecipitated from 1mg of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with ab205304 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205304 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
      Lane 1: U-87 MG whole cell lysate 10µg (Input).
      Lane 2: ab205304 IP in U-87 MG whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205304 in U-87 MG whole cell lysate.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 10 seconds.

    References

    ab205304 has not yet been referenced specifically in any publications.

    Publishing research using ab205304? Please let us know so that we can cite the reference in this datasheet.

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