Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)

Submit a review Submit a question

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17302] to Poliovirus Receptor/PVR
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Poliovirus Receptor/PVR antibody [EPR17302]
    See all Poliovirus Receptor/PVR primary antibodies

  • Description

    Rabbit monoclonal [EPR17302] to Poliovirus Receptor/PVR

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Poliovirus Receptor/PVR aa 200-350. The exact sequence is proprietary.
    Database link: P15151

  • Positive control

    • WB: HEK293T, HT1080, U-87 MG, A549, HUVEC and K562 whole cell lysates; human fetal heart, fetal kidney and fetal spleen lysates. IP: U-87 MG whole cell lysate.

  • General notes

     

    This product was previously labelled as Poliovirus Receptor

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17302

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab205304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa).
IP 1/30.

Target

  • Function

    Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.

  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD155 antibody
      • CD155 antigen antibody
      • FLJ25946 antibody
      • HVED antibody
      • mE4 antibody
      • NECL 5 antibody
      • Necl-5 antibody
      • Necl5 antibody
      • Nectin like 5 antibody
      • Nectin like protein 5 antibody
      • Nectin-like protein 5 antibody
      • Ortholog of mouse Tage4 antibody
      • Poliovirus receptor antibody
      • PVR antibody
      • PVR_HUMAN antibody
      • PVS antibody
      • Taa1 antibody
      • Tage 4 antibody
      • TAGE4 antibody
      see all

    Images

    • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293T cell lysate
      Lane 2 : PVR knockout HEK-293T cell lysate
      Lane 3 : Wild-type A549 cell lysate
      Lane 4 : PVR knockout A549 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 45 kDa
      Observed band size: 70 kDa
      why is the actual band size different from the predicted?



      Lanes 1- 4: Merged signal (red and green). Green - ab205304 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

       ab205304 was shown to react with Poliovirus Receptor/PVR in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266102 (knockout cell lysate ab257622) was used. Wild-type HEK-293T and PVR knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab205304 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunoprecipitation - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Immunoprecipitation - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)

      Poliovirus Receptor/PVR was immunoprecipitated from 1mg of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with ab205304 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205304 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
      Lane 1: U-87 MG whole cell lysate 10µg (Input).
      Lane 2: ab205304 IP in U-87 MG whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205304 in U-87 MG whole cell lysate.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 10 seconds.

    • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution

      Lane 1 : Wild-type A549 whole cell lysate
      Lane 2 : PVR knockout A549 whole cell lysate
      Lane 3 : U87-MG whole cell lysate
      Lane 4 : HUVEC whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 45 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab205304 observed at 70 kDa (ab205304), 60-80 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab205304 was shown to specifically react with Poliovirus Receptor in wild-type A549 cells as signal was lost in PVR knockout cells. Wild-type and PVR knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab205304 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/4000 dilution

      Lane 1 : HT1080 (Human fibrosarcoma cell line) whole cell lysate
      Lane 2 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
      Lane 3 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Predicted band size: 45 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution + K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Developed using the ECL technique.

      Predicted band size: 45 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : Human fetal spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 45 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Western blot - Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution

      Lane 1 : Untreated K562 (Human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
      Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate deglycosylation (PNGase F) treated

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 45 kDa
      Observed band size: 70 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)
      Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304)

    References (0)

    Publishing research using ab205304? Please let us know so that we can cite the reference in this datasheet.

    ab205304 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab205304.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com