Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17302] to Poliovirus Receptor/PVR - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Product nameAnti-Poliovirus Receptor/PVR antibody [EPR17302] - BSA and Azide free
See all Poliovirus Receptor/PVR primary antibodies
DescriptionRabbit monoclonal [EPR17302] to Poliovirus Receptor/PVR - BSA and Azide free
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Recombinant fragment aa 200-350. The exact sequence is proprietary.
Database link: P15151
- WB: HEK293T and A549 cell lysates. IP: U-87 MG cell lysate.
ab228348 is the carrier-free version of ab205304 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab228348 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Poliovirus Receptor
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
PurityProtein A purified
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab228348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 45 kDa).|
|IP||Use at an assay dependent concentration.|
FunctionMediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. Serves as a receptor for poliovirus attachment to target cells. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport.
Sequence similaritiesBelongs to the nectin family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- CD155 antibody
- CD155 antigen antibody
- FLJ25946 antibody
All lanes : Anti-Poliovirus Receptor/PVR antibody [EPR17302] (ab205304) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PVR knockout HEK-293T cell lysate
Lane 3 : Wild-type A549 cell lysate
Lane 4 : PVR knockout A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
This data was developed using the same antibody clone in a different buffer formulation (ab205304).
ab205304 was shown to react with Poliovirus Receptor/PVR in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266102 (knockout cell lysate ab257622) was used. Wild-type HEK-293T and PVR knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab205304 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Poliovirus Receptor/PVR was immunoprecipitated from 1mg of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate with ab205304 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205304 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: U-87 MG whole cell lysate 10µg (Input).
Lane 2: ab205304 IP in U-87 MG whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205304 in U-87 MG whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab205304).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228348 has not yet been referenced specifically in any publications.