Overview

  • Product name

  • Description

    Rabbit polyclonal to POLR1A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human POLR1A aa 817-862.
    Database link: O95602

  • Positive control

    • HEK293T, NIH-3T3 and H9C2 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab192389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 195 kDa.

Target

  • Function

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
  • Sequence similarities

    Belongs to the RNA polymerase beta' chain family.
  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • A190 antibody
    • AFDCIN antibody
    • DNA-directed RNA polymerase I largest subunit antibody
    • DNA-directed RNA polymerase I subunit A antibody
    • DNA-directed RNA polymerase I subunit rpa1 antibody
    • Polr1a antibody
    • Polymerase (RNA) I polypeptide A 194kDa antibody
    • RNA polymerase I 194 kDa subunit antibody
    • RNA polymerase I subunit A antibody
    • RNA polymerase I subunit A1 antibody
    • RPA1 antibody
    • RPA1_HUMAN antibody
    • RPA194 antibody
    • RPO1 4 antibody
    • RPO14 antibody
    see all

Images

  • All lanes : Anti-POLR1A antibody (ab192389)

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : NIH-3T3 whole cell lysate
    Lane 3 : H9C2 whole cell lysate

    Predicted band size: 195 kDa

References

ab192389 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab192389.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up